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master's thesis
Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika
2021. urn:nbn:hr:152:427708
Krstanović, Ines
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Krstanović, I. (2021). Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:427708

Krstanović, Ines. "Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:427708

Krstanović, Ines. "Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:427708

Krstanović, I. (2021). 'Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:152:427708

Krstanović I. Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:152:427708

I. Krstanović, "Učinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:427708

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TitleUčinak kratkotrajne dijete s povećanim udjelom NaCl na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika
Title (english)The effect of short-term diet with increased NaCl content on the frequencies of Th17 and Treg lymphocytes in the peripheral blood of healthy young subjects
AuthorInes Krstanović
MentorMartina Mihalj (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Odrediti zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika prije i nakon sedmodnevne dijete s povećanim udjelom kuhinjske soli. Nacrt studije: Studija je ustrojena kao nerandomizirani kontrolirani pokus u kojem su svi ispitanici bili podvrgnuti istom eksperimentalnom protokolu s više ponovljenih mjerenja. Ispitanici i metode: U istraživanju su sudjelovali zdravi mladi dobrovoljci, 10 žena i 10 muškaraca. Istraživanje je trajalo 14 dana te je bilo podijeljeno na sedmodnevno razdoblje NSD i sedmodnevno razdoblje VSD. Tijekom prvog razdoblja ispitanici su konzumirali 3,5 g soli, a tijekom drugog 14 g soli dnevno. Nakon svakog razdoblja ispitanicima je uzorkovana periferna venska krv iz koje su na gradijentu fikola izolirane mononuklearne stanice. Stanice su do analize pohranjene na - 80 °C. Za odmrzavanje uzoraka koristio se RPMI-1640 medij uz dodatak FBS-a i antibiotika. Dio stanica korišten je za imunofenotipizaciju Treg stanica. Iz preostalog uzorka izdvojeni su ukupni Th limfociti metodom magnetskog sortiranja pomoću komercijalno dostupnog seta (MagniSortTM Human CD4+ T cell Enrichment kit). Za poliklonsku aktivaciju Th stanica korišten je komercijalni set koji sadrži PMA i ionomicin (eBioscienceTM Cell Stimulation Cocktail). Naposljetku je slijedila imunofenotipizacija Th17 limfocita pomoću BD FACS Canto II citometra. Za statističku analizu korišten je program Graph Pad Prism v6.01 (GraphPad Software, San Diego, CA, USA). Rezultati: Nema značajne promjene u perifernim udjelima Treg i Th17 stanica nakon sedmodnevne VSD u odnosu na NSD. Zaključak: Kratkotrajna dijeta s povećanim udjelom kuhinjske soli ne utječe na zastupljenost Th17 i Treg limfocita u perifernoj krvi zdravih mladih ispitanika.
Abstract (english)
Objectives: To determine the presence of Th17 and Treg lymphocytes in the peripheral blood of healthy young subjects before and after a seven-day high-salt diet Study Design: The study was organized as a nonrandomized controlled experiment in which all subjects were subjected to the same experimental protocol with multiple replicate measurements. Participants and methods: Healthy young volunteers of both sexes (10 women and 10 men) participated in the study. The study lasted 14 days and included a 7-day period of low (NSD) and a 7-day period of high salt intake (VSD). In the first part of the study, subjects consumed 3,5 g of salt, while during the HSD intended salt intake was around 14 g per day. On days 7 and 14 of the protocol peripheral venous blood was sampled from which mononuclear cells were isolated on a ficol gradient. The cells were then stored at - 80 ° C until analysis. RPMI-1640 cell medium with the addition of FBS and antibiotics was used for cell recovery. Immediately after thawing, part of the cells were used for immunophenotyping of Treg cells, while remaining sample was used for total CD4+ helper T lymphocytes (Th) isolation by magnetic sorting using a commercially available set (MagniSortTM Human CD4+ T cell Enrichment). A commercial set containing PMA and ionomycin was used for polyclonal activation of Th cells (eBioscienceTM Cell Stimulation Cocktail). Finally, immunophenotyping of Th17 lymphocytes by BD FACS Canto II cytometer was performed. Statistical analysis was performed using Graph Pad Prism (v6.01, San Diego, CA, USA) Results: There was no significant change in peripheral Treg and Th17 cells frequencies after 7-day VSD compared to NSD. Conclusion: A short-term high-salt diet had no significant effect on Th17 and Treg lfrequencies in the peripheral blood of healthy young subjects. SUMMARY 26
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:427708
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-03-06 13:08:00