In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline

Feketija, Vedrana

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undergraduate thesis

In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline

2015. urn:nbn:hr:152:780176

Feketija, Vedrana

Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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APA 6th Edition

Feketija, V. (2015). In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:780176

MLA 8th Edition

Feketija, Vedrana. "In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2015. https://urn.nsk.hr/urn:nbn:hr:152:780176

Chicago 17th Edition

Harvard

Feketija, V. (2015). 'In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 21 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:780176

Vancouver

Feketija V. In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2015 [cited 2024 November 21] Available at: https://urn.nsk.hr/urn:nbn:hr:152:780176

IEEE

V. Feketija, "In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:152:780176

Cite this item: https://urn.nsk.hr/urn:nbn:hr:152:780176

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Metadata

Title	In vitro mikronukleus test u procjeni genotoksičnosti ksilena, metanola i ledene octene kiseline
Title (english)	In vitro micronucleus test as a tool of genotoxicity asessment of ksylen, methanol and icy acetic acid
Author	Vedrana Feketija
Mentor	Jasenka Wagner (mentor)
Committee member	Marija Heffer (član povjerenstva)
Committee member	Martina Smolić (član povjerenstva)
Committee member	Jasenka Wagner (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Faculty of Medicine Osijek Osijek
Defense date and country	2015-09-14, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Medical Biochemistry
Abstract	Mikronukleus (MN) test na limfocitima periferne krvi jedna je od najvaţnijih metoda koje se primjenjuju u biološkom nadzoru, a zasniva se na na 72-satnome uzgoju limfocita periferne krvi u hranjivom mediju te izradi mikroskopskih preparata koji se pregledavaju pod svjetlosnim mikroskopom. Cilj ovog istraţivanja je ispitati genotoksični učinak ksilola, metanola i ledene octene kiseline i odrediti indeks proliferacije limfocita periferne krvi zaustavljanjem citokineze pri različitim koncentracijama kemikalija mikronukleus testom. Analizirani su limfociti triju ispitanika tretirani s tri različite koncentracije svake od navedenih kemikalija. Biljeţen je ukupan broj MN, ukupni broj stanica s MN, raspodjela MN u stanicama, broj nukleoplazmatskih mostova (NPB) i nuklearnih pupova (Nbud) te indeks proliferacije stanica. Ksilol, metanol i ledena octena kiselina uzrokuju statistički značajno povećani broj mikronukleusa i smanjenje indeksa proliferacije (t test, P<0,05). Pokazano je da je mikronukleus test vrlo dobar pokazatelj genotoksičnog i citotoksičnog djelovanja ispitivanih kemikalija.
Abstract (english)	The micronucleus (MN) test on lymphocytes of peripheral blood is one of most important methods used in biologic monitoring. It is based on the 72-hour cultivation of lymphocytes of peripheral blood in nutrient medium and making microscopic preparations which are observed under optical microscope. The aim of this research was to examine the genotoxic effect of xylene, methanol and glacial acetic acid, and to determine the proliferation index of peripheral blood lymphocytes by cytokinesis at different chemical concentrations in the micronucleus test. The lymphocytes of three participants are analyzed with three different concentrations of each of the enlisted chemicals. The total number of MN, the total number cells with MN, the distribution of MN in cells, the number of nucleoplasmatic bridges (NPB) and nuclear buds (Nbud) and cell proliferation index were recorded. Xylene, methanol and glacial acetic acid cause a significantly increased number of micronuclei and lowered proliferation index (t test, P<0,05). It was proven that micronucleus test is a very good indicator of genotoxic and cytotoxic action of the tested chemicals.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:152:780176
Study programme	Title: University undergraduate study; specializations in: medical laboratory diagnostics Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2016-05-05 10:04:04