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master's thesis
Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora
2019. urn:nbn:hr:152:712742
Blažević, Martina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Blažević, M. (2019). Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:712742

Blažević, Martina. "Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:712742

Blažević, Martina. "Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:712742

Blažević, M. (2019). 'Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:712742

Blažević M. Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:712742

M. Blažević, "Utjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:712742

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TitleUtjecaj kratkotrajno povećanog unosa kuhinjske soli na udio regulatornih T limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora
Title (english)The effect of short term high salt diet on frequencies of nTreg cells in peripheral blood and mesenteric lymph nodes of Sprague-Dawley rats
AuthorMartina Blažević
MentorMartina Mihalj (mentor)
Committee memberStana Tokić (predsjednik povjerenstva)
Committee memberAnita Matić (član povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraţivanja: Ispitati učinak kratkotrajne (7 i 14 dana) dijete s povećanim unosom soli na udio regulatornih CD4 T-limfocita u perifernoj krvi i mezenteričnim limfnim čvorovima Sprague-Dawley štakora. Nacrt studije: Pokusno (eksperimentalno) istraţivanje Materijali i metode: Sprague-Dawley štakori starosti od šest do devet tjedana podijeljeni su u tri skupine (N = 6 – 9). Prva skupina ţivotinja bila je izloţena visokoslanoj dijeti tijekom sedam dana (VSD-7,4 % NaCl, Mucedola, Italija), druga skupina ţivotinja bila je na visokoslanoj dijeti 14 dana (VSD-14), a treća skupina ţivotinja bila je hranjena standardnom laboratorijskom hranom (KONTROLA). Prikupljene stanice iz uzoraka periferne krvi i mezenteričnih limfnih čvorova analizirane su protočnim citometrom (BD FACS Canto II). Rezultati: Pokazano je da dijeta s visokim udjelom soli dovodi do statistički značajnih promjena u frekvencijama T regulatornih limfocita. Nakon sedmodnevne i četrnaestodnevne visokoslane dijete frekvencija Treg stanica u perifernoj krvi štakora je bila statistički značajno sniţena u odnosu na udio Treg stanica u kontrolnoj skupini, dok je frekvencija Treg stanica u mezenteričnim limfnim čvorovima takoĎer bila sniţena u odnosu na udio Treg stanica kontrolne skupine, ali bez statističke značajnosti. Zakljuĉak: Povećani unos soli dovodi do smanjenja frekvencije T regulatornih limfocita što ukazuje da visokoslana dijeta djeluje na smanjenje broja Treg stanica u Sprague-Dawley štakora.
Abstract (english)
Objectives: The affect of short term (7 and 14 days) high salt diet on frequencies of nTreg cells in peripheral blood and mesenteric lymph nodes of Sprague-Dawley rats. Study draft: Experimental research Materials and methods: Sprague-Dawley rats, age 6-9 weeks diveded into three groups (N=6-9). The first group of animals was on a high-salt diet for 7 days (HSD-7,4 % NaCL, Murcedola , Italy), the second group of animals was on the same diet for 14 days( HSD-14) and the third group was fed on standard lab diet (CONTROL). Collected peripheral blood and mesentric lymph nodes sample cells were analysed by flow cytometer. Results: It is indicated that high-salt diet leads to statistically significant changes in T regulatory lymphocytes frequencies. After 7 and 14-days high-salt diet the frequency of regulatory T cells in peripheral rat blood was statistically significantly reduced comparing to ratio of Treg cells of the control group while the frequency in mesentric lymph nodes was also reduced comparing to Treg cells ratio of the control group but with no statistical significance. Conclusion: Excessive salt consumption leads to reduction of Treg cell frequency which indicates that high-salt diet affects Treg cell numbers in Sprague-Dawley rats.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:712742
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-06 09:51:15