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master's thesis
Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije
2019. urn:nbn:hr:152:696586
Zucić, Jasminka
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Zucić, J. (2019). Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:696586

Zucić, Jasminka. "Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:696586

Zucić, Jasminka. "Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:696586

Zucić, J. (2019). 'Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:696586

Zucić J. Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:696586

J. Zucić, "Promjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:696586

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TitlePromjene proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora pod utjecajem tretmana hiperbarične oksigenacije
Title (english)Changes in protein expression level of antioxidative enzymes in brain blood vessels of Sprague-Dawley rats under the influence of hyperbaric oxygenation treatment
AuthorJasminka Zucić
MentorAnita Matić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberAnita Matić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
CILJ ISTRAŽIVANJA: Cilj je istraživanja bio procijeniti učinke akutne i povremene hiperbarične oksigenacije na proteinski izražaj antioksidativnih enzima u krvnim žilama mozga. NACRT STUDIJE: Eksperimentalna studija na pokusnim laboratorijskim životinjama. ISPITANICI I METODE: Zdravi muški Sprague-Dawley štakori starosti 9 – 11 tjedana nasumce su razvrstani u skupine (n = šest štakora/skupina) – kontrolnu (intaktne životinje) i dvije hiperbarične skupine koje su izložene jednom tretmanu hiperbarične oksigenacije (akutna; A-HBO2) ili intermitentnu skupinu (četiri uzastopna HBO2 tretmana, 4D- HBO2). Svaka je životinja izvagana te su prikupljene krvne žile mozga u svrhu određivanja proteinskog izražaja antioksidativnih enzima (Cu/Zn SOD, MnSOD, EC-SOD, GPx4 i katalaze) Western blot metodom. REZULTATI: Nije utvrđena statistički značajna razlika u tjelesnoj masi između ispitivanih skupina (p = 0,411). Hiperbarična oksigenacija (neovisno o protokolu terapije) dovodi do značajne razlike u proteinskom izražaju Cu/Zn SOD, MnSOD, GPx4 i katalaze u odnosu na kontrolnu. Proteinski izražaj katalaze i GPx4 značajno je povećan u akutnoj ispitivanoj skupini u odnosu na ostale ispitivane skupine. Cu/Zn SOD izražaj pokazao se najveći u kontrolnoj skupini, a jedino je proteinski izražaj MnSOD značajno povećan kod intermitentne oksigenacije u odnosu na druge skupine. ZAKLJUČAK: Snižena razina oksidacijskog stresa kod intermitentne hiperbarične oksigenacije posljedica je, promatranjem proteinske razine, povećanja izražaja MnSOD antioksidativnog enzima.
Abstract (english)
RESEARCH OBJECTIVES: The aim of the study is to assess the effect of acute and intermittent hyperbaric oxygenation on the protein expression of antioxidative enzymes in brain blood vessels. STUDY DESIGN: Experimental study on laboratory animals (Sprague-Dawley rats) METHODS: 9-11 weeks old healthy male Sprague-Dawley rats were randomly assigned to groups (n = 6 rats/groups): control group (intact animals) and 2 hyperbaric groups that underwent single (acute; A-HBO2) hyperbaric oxygenation treatment or intermittent group (4 consecutive HBO2 treatments, 4d HBO2). Every animal was weighed and brain blood vessels were collected for the purpose of determining the protein expression of the antioxidative enzymes (Cu/Zn SOD, MnSOD, EC-SOD, GPx4 and catalase) with Western Blot method. RESULTS: Statistically significant difference in body mass was not determined between the groups (p = 0,411). Hyperbaric oxygenation (regardless of therapy protocol) leads to a significant difference in protein expression of Cu/Zn SOD, MnSOD, GPx4, and catalase compared to the control group. The catalase protein expression, as well as the GPx4, were significantly increased in the A-HBO2 group compared to the other tested groups. Cu/Zn SOD expression was the largest in the control group, whereas the protein expression MnSOD was only increased significantly by intermittent oxygenation compared to the other tested groups. CONCLUSION: Having observed the protein levels, we can conclude that the lowered level of oxidative stress by intermittent hyperbaric oxygenation is the result of antioxidative enzyme MnSOD’s increased expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:696586
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-07 11:31:37