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Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora
2019. urn:nbn:hr:152:863991
Spudić, Ivona
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Spudić, I. (2019). Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:863991

Spudić, Ivona. "Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:863991

Spudić, Ivona. "Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:863991

Spudić, I. (2019). 'Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 October 2024, https://urn.nsk.hr/urn:nbn:hr:152:863991

Spudić I. Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 October 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:863991

I. Spudić, "Učinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:863991

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TitleUčinak hiperbarične oksigenacije na razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi i izražaj gena za antioksidativne enzime u krvnim žilama Sprague-Dawley štakora
Title (english)The effect of hyperbaric oxygenation (HBO2) on the level of oxydative stress in peripherl blood mononuclear cells and antioxidative enzyme gene expression in blood vessels of Sprague-Dawley rats
AuthorIvona Spudić
MentorMartina Mihalj (mentor)
Committee memberStana Tokić (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
CILJ ISTRAŽIVANJA: Cilj istraživanja bio je utvrditi razinu oksidativnog stresa u mononuklearnim stanicama periferne krvi zdravih muških Sprague-Dawley štakora izloženih akutnoj i intermitentnoj hiperbaričnoj oksigenacij (HBO2) te izražaj antioksidativnih enzima u krvnim žilama mozga istih životinja. NACRT STUDIJE: Eksperimentalna studija na pokusnim laboratorijskim životinjama ISPITANICI I METODE: Istraživanje je provedeno na zdravim Sprague-Dawley štakorima muškog spola u starosti od 9 do 11 tjedana. Životinje su bile podijeljene u 3 skupine (n = 6-8): (1) životinje podvrgnute akutnoj hiperbaričnoj oksigenaciji (A-HBO2); (2) životinje podvrgnute intermitentnoj HBO2 tijekom 4 uzastopna dana (4D-HBO2) te (3) skupina zdravih štakora koji nisu bili izloženi HBO2. Izražaj gena za izoforme superoksid dismutaze (SOD), izoforme gluation dismutaze (GPx) te katalazu (CAT) u izoliranim moždanim žilama, određen je pomoću PCR-a u stvarnom vremenu, a razina oksidativnog stresa u mononuklearnim stanicama iz periferne krvi procijenjena je bojanjem stanica diklorofluorescein diacetatom (DCF-DA) i analizom na protočnom citometru. REZULTATI: Akutno izlaganje životinja HBO2 dovodi do značajnog smanjenja izražaja MnSOD, GPx1, GPx i katalaze, dok intermitentno izlaganje dovodi do normalizacije njihovog izražaja. U slučaju EC-SOD te GPx4, akutno izlaganje HBO2 dovelo je do porasta njihovih izražaja te ono ostaje na istoj značajno višoj razini i nakon intermitentnog izlaganja tijekom 4 dana. U mononuklearnim stanicama periferne krvi bazična razina oksidativnog stresa, kao i ona nakon stimulacije stanica s PMA, nije se mijenjala nakon akutne hiperbarične oksigenacije, dok nakon intermitentnog izlaganja zamjećujemo pad razine oksidativnog stresa. Međutim, razlike nisu bile statistički značajne. ZAKLJUČAK: Rezultati našeg istraživanja sugeriraju da intermitentno izlaganje Sprague-Dawley štakora hiperbaričnom kisiku dovodi do promjene u izražaju oksidativnih enzima, što može rezultirati smanjenom razinom slobodnih radikala u mononuklearnim stanicama periferne krvi.
Abstract (english)
RESEARCH OBJECTIVE: The aim of the study was to determine the level of oxidative stress in the peripheral mononuclear blood cells of healthy male Sprague-Dawley rats exposed to acute and intermittent hyperbaric oxygenation, (HBO2) and expression of antioxidant enzymes in the blood vessels of the same animals. STUDY DESIGN: Experimental study on laboratory animals MATHERIALS AND METHODS: The study was conducted on healthy Sprague-Dawley male rats at 9 to 11 weeks of age. Animals were divided into 3 groups (n = 6-8): (1) animals undergoing acute hyperbaric oxygenation (A-HBO2); (2) animals subjected to intermittent HBO2 for 4 consecutive days (4D-HBO2) and (3) a group of healthy rats that were not exposed to HBO2. Gene expression for superoxide dismutase (SOD) isoforms, gluation dismutase isoforms (GPx) and catalase (CAT) in isolated cerebellum was determined by real- time PCR, and the level of oxidative stress in peripheral mononuclear blood cells was estimated by cell staining dichlorofluorescein diacetate (DCF-DA) and flow cytometer analysis. RESULTS: Acute exposure of HBO2 animals leads to a significant decrease in the expression of MnSOD, GPx1, GPx and CAT, while intermittent exposure leads to a normalization of their expression. In the case of EC-SOD and GPx4, acute exposure to HBO2 led to an increase in their expression and remained at the same significantly higher level after intermittent exposure for 4 days. In peripheral mononuclear blood cells, the baseline level of oxidative stress, as well as that after stimulation of cells with PMA, did not change after acute hyperbaric oxygenation, whereas after intermittent exposure, a decrease in the level of oxidative stress was observed. However, the differences were not statistically significant. CONCLUSION: The results of our study suggest that intermittent exposure of Sprague- Dawley rats to hyperbaric oxygen leads to a change in the expression of oxidative enzymes, which can result in reduced levels of free radicals in peripheral mononuclear blood cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:863991
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-07 12:08:19