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undergraduate thesis
Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.
2020. urn:nbn:hr:152:370284
Grgić, Zvonimir
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Grgić, Z. (2020). Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze. (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:370284

Grgić, Zvonimir. "Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:370284

Grgić, Zvonimir. "Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:370284

Grgić, Z. (2020). 'Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 18 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:370284

Grgić Z. Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze. [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2024 December 18] Available at: https://urn.nsk.hr/urn:nbn:hr:152:370284

Z. Grgić, "Uloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:370284

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Metadata
TitleUloga perifernih CD3+CD8+ limfocita T u pojavi i progresiji vulgarne psorijaze.
Title (english)The role of peripheral CD3+ CD8+ T lymphocytes in initiation and progression of psoriasis vulgaris.
AuthorZvonimir Grgić
MentorStana Tokić (mentor)
Committee memberMario Štefanić (predsjednik povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
Committee memberStana Tokić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-07-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Vulgarna psorijaza je kronična bolest kože posredovana združenim djelovanjem rizičnih okolišnih čimbenika i nekontroliranog imunološkog odgovora na nepoznate antigene. Velik udio upalnog leukocitnog infiltrata čine CD8+ citotoksični limfociti T koji lučenjem IL-17/IFN-γ/TNF-α potiču razvoj upalnog mikrookoliša u koži, proliferaciju i dediferencijaciju keratinocita i posljedični razvoj ljuskavih plakova. Cilj istraživanja: Analizirati i usporediti brojnost CD3+CD8+ limfocita T u oboljelih i zdravih ispitanika te ustanoviti povezanost njihove promjene s demografskim i kliničkim obilježjima psorijaze. Materijali i metode: Istraživanjem su obuhvaćeni uzorci periferne krvi 22 zdrava (CTRL) i 21 oboljelog (PV) ispitanika. Periferni leukociti izolirani su centrifugiranjem u gradijentu Lymphoprep™ medija. Mrtve stanice isključene su primjenom boje za određivanje vijabilnosti stanica („LIVE/DEAD™ Fixable Dead Cell Stain Kits“), a imunofenotipizacija CD3+CD8+ stanica provedena protočnom citometrijom (BD FACS Canto II) nakon obilježavanja s anti-human CD3 FITC i CD8 PerCP monoklonalnim protutijelima. FlowLogic računalni program korišten je za obradu podataka. Rezultati: U odnosu na kontrolne ispitanike, oboljeli od PV pokazuju značajno smanjene udjele CD3+CD8+ limfocita T u perifernoj krvi čiji su udjeli značajno veći u CMV seropozitivnih nego CMV seronegativnih ispitanika. Zaključak: U odnosu na zdrave ispitanike, PV oboljeli imaju značajno snižene udjele CD3+CD8+ limfocita T u perifernoj krvi na čiju brojnost značajno utječu prethodne CMV infekcije.
Abstract (english)
Introduction: Psoriasis vulgaris (PV) is a chronic skin disease, mediated by environmental risk factors and aberrant immune response to unknown antigens. CD8+ cytotoxic lymphocytes make a large proportion of the leukocyte infiltrate. Their secretion of IL-17/IFN-γ/TNF-α helps in growing inflammatory state in a skin, keratinocytes proliferation and dedifferentiation and consequently, forming scaly plaques. Objectives: To examine and compare frequency of CD3+CD8+ T lymphocytes in PV patients and healthy controls and determine their association with demographic and clinical features of psoriasis. Material and methods: Peripheral blood was collected from 21 PV patients and 22 healthy controls. Peripheral leukocytes were isolated during centrifugation in density gradient Lymphoprep™ medium. Dead cells were excluded with „LIVE/DEAD™ Fixable Dead Cell Stain Kit“. Immunophenotyping CD3+CD8+ T lymphocytes was performed on a flow cytometry (BD FACS Canto II) after labeling samples with anti-human CD3 FITC and CD8 PerCP monoclonal antibodies. FlowLogic software was used for data analysis. Results: Compared to the control subjects, patients with PV showed significantly lower frequency of CD3+CD8+ T lymphocytes in peripheral blood, whose frequencies are significantly increased in CMV seropositive subjects. Conclusion: Compared to healthy subjects, PV patients have had significantly decreased frequencies of CD3+CD8+ T lymphocytes in peripheral blood, whose frequency is fairly affected by former CMV infections.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:370284
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-11-12 11:53:17