prikaz prve stranice dokumenta Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa
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undergraduate thesis
Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa
2020. urn:nbn:hr:152:681256
Atlagić, Borna
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Atlagić, B. (2020). Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:681256

Atlagić, Borna. "Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:681256

Atlagić, Borna. "Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:681256

Atlagić, B. (2020). 'Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 September 2023, https://urn.nsk.hr/urn:nbn:hr:152:681256

Atlagić B. Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2023 September 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:681256

B. Atlagić, "Analiza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:681256

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TitleAnaliza modificirane metode deparafiniranja histoloških preparata otopinom deterdženta za pranje posuđa
Title (english)Analysis of a modified deparaffinization method for histological slides using a dish-washing solution
AuthorBorna Atlagić
MentorTatjana Belovari (mentor)
Committee memberKsenija Marjanović (predsjednik povjerenstva)
Committee memberBiljana Pauzar (član povjerenstva)
Committee memberTatjana Belovari (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
CILJ ISTRAŽIVANJA: Modificirati metodu deparafiniranja 1,7 %-tnom otopinom deterdženta za pranje posuđa korištenjem nižih temperatura u različitom vremenu, ocijeniti efikasnost deparafiniranja modificiranom metodom te usporediti kvalitetu histoloških preparata živčanog tkiva deparafiniranih modificiranom metodom i poznatom metodom deparafiniranja 1,7 %-tnom otopinom DPP-a. USTROJ STUDIJE: Presječna studija. MATERIJALI I METODE: Istraživanje je provedeno na uzorcima iz arhive Kliničkog zavoda za patologiju i sudsku medicinu KBC Osijek. Upotrijebljeno je 10 uzoraka živčanog tkiva uklopljenog u parafin. Rezovi svakog uzorka podijeljeni su u 5 skupina: četiri skupine koje su deparafinirane 1,7 %-tnom otopinom DPP-a na temperaturi od 60 °C ili 70 °C, tijekom jednoga ili dva sata i kontrolna skupina koja je deparafinirana 1,7 %-tnom otopinom DPP-a na 90 °C. Dobiveni rezovi obojeni su hemalaunom-eozinom i histološki analizirani. REZULTATI: Analizom kvalitete dobivenih preparata utvrđeno je da ne postoji značajna razlika u kvaliteti između pokusnih skupina deparafiniranih na 60 °C i kontrolne skupine. Uzorci deparafinirani na 70 °C bili su značajno slabije kvalitete od kontrolne skupine. Produženo vrijeme deparafiniranja, neovisno o temperaturi, dovelo je do većeg gubitka uzoraka tijekom postupka. ZAKLJUČAK: Uzorci deparafinirani na temperaturi od 60 °C bili su podjednake kvalitete kao i kontrolni, te bi se ova metoda pripreme u nekim slučajevima mogla koristiti kao zamjena originalnoj metodi. Uzorci deparafinirani na temperaturi od 70 °C bili su značajno slabije kvalitete u odnosu na kontrolu.
Abstract (english)
OBJECTIVES: To modify the 1.7 % dishwashing solution deparaffinization process by using lower temperatures in a different time period in order to evaluate the efficacy of deparaffinization with the modified method and to compare the quality of neural tissue slides deparaffinized with the modified method and the established 1,7 % solution deparaffinization method. STUDY DESIGN: Cross-sectional study. MATERIAL AND METHODS: The study was conducted on samples obtained from the Clinical Institute of Pathology and Judicial Medicine, University Hospital Centre Osijek. 10 samples of neural tissue were used and embedded into paraffin. The slides from all samples were divided into 5 groups: four groups deparaffinized with 1.7 % solution at 60 °C or 70 °C during 1 hour or 2 hours and the control group which was deparaffinized with 1,7 % solution at 90 °C. The acquired slides were stained with hematoxylin-eosin and histological analysis was performed. RESULTS: By analysis of the obtained slides it was observed that there was no significant difference in quality between the experimental groups deparaffinized at 60 °C and the control group. The samples deparaffinized at 70 °C were of a significantly lower quality than the control group. Longer deparaffinization time, regardless of temperature, led to more sample loss during the process. CONCLUSION: The samples deparaffinized at 60 °C were of similar quality as the control samples. Therefore, in some cases this modified method could be used as a substitute for the original method. Samples deparaffinized at 70 °C were of significantly lower quality when compared to the controls.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:681256
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-12-15 13:49:14