OBJECTIVES: To modify the 1.7 % dishwashing solution deparaffinization process by using lower temperatures in a different time period in order to evaluate the efficacy of deparaffinization with the modified method and to compare the quality of neural tissue slides deparaffinized with the modified method and the established 1,7 % solution deparaffinization method. STUDY DESIGN: Cross-sectional study. MATERIAL AND METHODS: The study was conducted on samples obtained from the Clinical Institute of Pathology and Judicial Medicine, University Hospital Centre Osijek. 10 samples of neural tissue were used and embedded into paraffin. The slides from all samples were divided into 5 groups: four groups deparaffinized with 1.7 % solution at 60 °C or 70 °C during 1 hour or 2 hours and the control group which was deparaffinized with 1,7 % solution at 90 °C. The acquired slides were stained with hematoxylin-eosin and histological analysis was performed. RESULTS: By analysis of the obtained slides it was observed that there was no significant difference in quality between the experimental groups deparaffinized at 60 °C and the control group. The samples deparaffinized at 70 °C were of a significantly lower quality than the control group. Longer deparaffinization time, regardless of temperature, led to more sample loss during the process. CONCLUSION: The samples deparaffinized at 60 °C were of similar quality as the control samples. Therefore, in some cases this modified method could be used as a substitute for the original method. Samples deparaffinized at 70 °C were of significantly lower quality when compared to the controls.