OBJECTIVES: Genotyping of pharmacogenetic polymorphisms rs1799853 (*2) and rs1057910 (*3) of the CYP2C9 gene and determination of the frequency of allelic variants and genotypes of CYP2C9 for the population of east Croatia. STUDY DESIGN: Cross-sectional study. PARTICIPANTS AND METHODS: Group of participants consisted of 70 voluntary blood donors (56 men, 14 women) whose blood sample was collected in the period from March 2015 to March 2017 at the Clinical Institute of Transfusion Medicine, University Hospital Osijek. Genomic DNA was isolated from whole blood samples with EDTA anticoagulant. Before genotyping, purity and concentration of DNA were determined spectrophotometrically. Genotyping of CYP2C9*2 and CYP2C9*3 was performed by real-time PCR with high-resolution melting curve analysis. The frequency of CYP2C9 alleles and genotypes was compared with previously published results for other populations. Statistical program MedCalc was used for statistical analysis. RESULTS: In the group of 70 participants, 48 participants (69 %) were homozygotes for wild type *1/*1, 12 participants (17 %) had genotype *1/*2, 7 participants (10 %) had genotype *1/*3, 2 participants (3 %) had genotype *2/*3, 1 participant (1 %) was homozygous *3/*3, while no one of the participants had the genotype *2/*2. The allelic frequency of CYP2C9*1 was 82 %, CYP2C9*2 10 % and CYP2C9*3 8 %. CONCLUSION: In the population of east Croatia, the most common is the allelic variant CYP2C9*1 and the genotype CYP2C9*1/*1. No statistically significant difference was observed between the population of east Croatia and the populations of northern India, Jordan, Italy, Croatia, Greece and Romania. There is a statistically significant difference between the population of eastern Croatia and the populations of Kosovo and China.