Objectives: To test the level of protection of privacy, awareness and risk behavior on the Internet among undergraduate students of Medical Laboratory Diagnostics considering their gender and age. Study Design: Study was conducted as a cross-sectional study. Participants and Methods: 59 undergraduate students participated in this study. The study was conducted at the Faculty of Medicine in Osijek during May 2019. The validated questionnaire was authored by Krešimir Šolić and Tena Velki. Statistical data was processed in the MedCalc computer program. Results: The majority of participants answered the general questions about safety and knowledge about the Internet, with an average grade being “good”. As many as 25.4% of respondents stated that they had not had any education on online security issues up until then. Respondents showed relatively high scores on all scales measured. Women have proven to be significantly more aware of the risks that are present on the Internet. The younger group of respondents, between 18 and 21 years old, showed a significant difference in risk awareness than the older group, between 22 and 25 years old. No statistically significant differences between genders or age were found in the behavioral and knowledge scales. Only 5.1% of respondents revealed their real password to trick question. Conclusion: Although most respondents showed high scores on all scales, this does not mean that the results are satisfactory. The highest scores were given on the behavioral scale, indicating that respondents are cautious in their actions. On the other hand, the lowest scores are on the awareness scale indicating a lack of education. The results obtained are not surprising compared to previous research, but there is a great need for additional education and awareness among Internet users.