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undergraduate thesis
Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina
2019. urn:nbn:hr:152:413015
Kuzmić, Matija
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kuzmić, M. (2019). Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:413015

Kuzmić, Matija. "Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:413015

Kuzmić, Matija. "Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:413015

Kuzmić, M. (2019). 'Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:413015

Kuzmić M. Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:152:413015

M. Kuzmić, "Određivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:413015

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Metadata
TitleOdređivanje promjene u ekspresiji gena CAIX u tumorskim stanicama izloženim derivatima kumarina
Title (english)Determination of changes in CAIX gene expression in cells exposed to coumarine derivatives
AuthorMatija Kuzmić
MentorTeuta Opačak-Bernardi (mentor)
Committee memberStana Tokić (predsjednik povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Uvod: Karboanhidraza IX, kao jedan od ključnih enzima u procesu održavanja acido-bazne ravnoteže tumora, predstavlja ciljano središte protutumorske terapije. Inhibicijom njezinog aktivnog mjesta omogućena je kontrola regulacije stvaranja potrebnog mikrookoliša za daljnju proliferaciju tumorskih stanica. U ovom su istraživanju za inhibiciju CAIX korišteni derivati kumarina NS48, NS51 i A153. Kumarin je zbog svoje karakteristične aktivnosti dobar inhibitor aktivnog mjesta CAIX. Cilj: Cilj je istraživanja odrediti utjecaj kumarinskih derivata na gensku ekspresiju CAIX u stanicama adenokarcinoma grlića maternice i stanicama karcinoma debelog crijeva. Materijali i metode: Stanične linije korištene u istraživanju su HeLa stanice adenokarcinoma grlića maternice i Caco-2 stanice karcinoma debelog crijeva. Stanice su tretirane kumarinskim derivatima NS48, NS51 i A153. Nakon kultivacije, RNA je izolirana i ekspresija određena qRT-PCR-om. Vrijednosti dobivene qRT-PCR reakcijom obrađene su ΔΔCT metodom kako bi se kvantificirala razina genske ekspresije. Rezultati: Tretman stanica adenokarcinoma grlića maternice, kao i stanica karcinoma debelog crijeva, kumarinskim derivatima pokazuju povećanje genske ekspresije za CAIX. Uzrok povećanja genske ekspresije pretpostavka je da je došlo do blokiranja aktivnog mjesta enzima navedenim derivatima. Zaključak: Kumarinski derivati NS48, NS51 i A153 uzrokuju povećanje ekspresije gena za CAIX u svim ispitivanim uzorcima.
Abstract (english)
Introduction: Carbonic anhydrase IX, as one of the key enzymes in the process of maintenance of acid-base balance of tumors, is the center target for anti-cancer therapy. Inhibition of its' active site enables the regulation of the micro environment which prevents further proliferation of tumor cells. In this study the compounds used to inhibit CAIX are derivatives of coumarin, NS48, NS51 and A153. Coumarin is a potent inhibitor of CAIX active site and thus affect the change in the level of gene expression for CAIX. Goals: The goal is to determine the effect of coumarin derivatives on gene expression for CAIX in cervical adenocarcinoma and colon cancer cell lines Materials and methods: The cell lines used in the study are HeLa cells of cervical adenocarcinoma and Caco-2 cells of colon cancer. The cells were treated with coumarin derivates NS48, NS51 and A153. After cultivation, RNA was isolated from each culture as well as from the controls, and further tested by qRT-PCR. The values obtained by qRT-PCR reaction were analysed with the ΔΔCT method to calculate the level of gene expression. Results: Cervical adenocarcinoma cells, as well as colon cancer cells treated with coumarin derivates, have shown increased gene expression of CAIX. Conclusion: The NS48, NS51 and A153 coumarin derivates increase the expression of CAIX in the cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:413015
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-05-10 08:47:31