Introduction: The process of myelination produces the myelin sheath, which is made by oligodendrocytes in the CNS. It is composed of proteins (MBP, PLP, DM20, CNP, MOG and MAG) and a large amount of lipids (glyphophospholipids and cholesterol dominate). Lipid rafts, high-order domains whose main organizers are gangliosides, play a role in the formation of the myelin membrane. Goals: To determine the distribution of complex gangliosides and myelin markers before, during, and after completion of myelination in the brain of a wild type of mouse C57Bl6 Materials and Methods: Wild-type mice aged 5, 15 and 30 days were used. After genotyping, the animals were sacrificed and the brains isolated, fixed and cryoprotected. Immunohistochemical analysis was performed using CNPase, MAG, MBP, GM1, GD1a, GD1b and GT1b antibodies at 35µm sections. The results were observed with a light microscope, photographed, analyzed in Fiji, and statistics was made in Statistica v22. Results: Considering the age, a statistically significant difference was found in all areas, for all myelin markers except MBP in the ICP area. There was a statistically significant difference between CC and ICP for all markers at all ages. In complex gangliosides, there is an apparent change between brains which were 5 days old compared to brains which were 15 and 30 days old. There are differences between 15 and 30 days old mice, but they are not statistically significant. Conclusion: The distribution of complex gangliosides on myelin sheaths increases during the myelination process in the brain of a wild type of mouse C57Bl6. The expression of myelin markers was statistically different in mice which were 5 days old compared to mice which were 15 and 30 days old.