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master's thesis
Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora
2020. urn:nbn:hr:152:399682
Jakšić, Petra
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Jakšić, P. (2020). Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:399682

Jakšić, Petra. "Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:399682

Jakšić, Petra. "Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:399682

Jakšić, P. (2020). 'Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 13 March 2023, https://urn.nsk.hr/urn:nbn:hr:152:399682

Jakšić P. Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2023 March 13] Available at: https://urn.nsk.hr/urn:nbn:hr:152:399682

P. Jakšić, "Utjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:399682

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TitleUtjecaj intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod zdravih Sprague-Dawley štakora
Title (english)Effects of intermittent hyperbaric oxygenation on flow-induced dilation in healthy Sprague-Dawley rats
AuthorPetra Jakšić
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Glavni cilj ovog istraživanja je odrediti učinak intermitentne hiperbarične oksigenacije na protokom potaknutu dilataciju kod Sprague-Dawley štakora. Materijali i metode: U istraživanje su bili uključeni zdravi muški Sprague-Dawley (SD) štakori podijeljeni u dvije skupine: zdravi netretirani štakori (CTRL, N = 12) te štakori izloženi intermitentnoj hiperbaričnoj oksigenaciji (100 % O2, 2h/2 bara) tijekom četiri uzastopna dana, a potom bili žrtvovani 5.dan (4D-HBOT, N = 11). Štakori su bili anestezirani ketaminom (75 mg/kg) i midazolamom (0,5 mg/kg). Nakon dekapitacije središnja moždana arterija je bila izolirana te postavljena u komoricu ispunjenu pripremljenom fiziološkom otopinom, a zatim inkubirana pri tlaku ∆80 mmHg radi procjene bazalnog promjera. Potom su se mjerile bazalne vrijednosti promjera žile pri tlačnim gradijentima koji su bili dobiveni istovremenim promjenama ulaznog i izlaznog tlaka (tlačni gradijenti Δ10, Δ20, Δ40, Δ60 i Δ100 mmHg). Nakon izmjerenih bazalnih vrijednosti, vazodilatacija ovisna o endotelu je bila ispitivana acetilkolinom (ACh), a o endotelu neovisnu natrijevim nitroprusidom (SNP) pri tlaku 80 mmHg (bez protoka). Na kraju protokola PSS je bila zamijenjena s PSS Ca-free otopinom radi procjene maksimalnog promjera krvne žile. Rezultati: Protokom potaknuta dilatacija središnjih moždanih arterija u eksperimentalnoj skupini životinja (4D-HBOT) i kontrolnoj skupini (CTRL) nije pokazala statistički značajnu razliku. Dodatkom acetilkolina (o endotelu-ovisan vazodilatator) te SNP-a (o endotelu neovisna vazodilatacija) nije utvrđena statistički značajna razlika u dilataciji kod 4D-HBOT u odnosu na CTRL. Dodatkom PSS Ca-free otopine također nije utvrđena statistički značajna razlika u protokom potaknutnoj dilataciji između CTRL i 4D-HBOT. Zaključak: Intermitentna hiperbarična oksigenacija ne mijenja odgovor izoliranih rezistentnih žila na protokom potaknutu dilataciju kod zdravih muških SD štakora, kao ni odgovor na acetilkolin ili SNP, odnosno o endotelu osisnu/neovisnu dilataciju.
Abstract (english)
Objective: The main aim of this study was to determine the effects of intermittent hyperbaric oxygenation on the flow-induced dilation in healthy Sprague-Dawley rats. Methods: Healthy male Sprague-Dawley (SD) rats were included in this research and were divided into two groups: healthy untreated rat (CTRL, N=12) and the rats exposed to intermittent hyperbaric oxygenation in a hyperbaric oxygen chamber (100 % O2, 2h/2 atm) over the course of 4 days and then they were sacrificed on the 5th day (4D-HBOT, N = 11). Rats were anesthetized (ketamine, 75 mg/kg and midazolam, 0,5 mg/kg) and afterwards decapitated. The middle cerebral artery was isolated and placed in a chamber filled with PSS solution, then incubated for 1 hour at pressure of ∆80 mmHg to estimate basal diameter. After that, basal diameter values were measured depending on the pressure gradients obtained by simultaneous changes of inlet and outlet pressure (pressure gradients Δ10, Δ20, Δ40, Δ60 i Δ100 mmHg). After the basal diameter values were determined, endothelium-dependent vasodilation was assayed with acetylcholine and independent vasodilation with SNP at the pressure of 80 mmHg without flow. In the end, PSS solution was replaced by PSS Ca-free and the maximum blood vessel diameter was measured. Results: Flow-induced dilation in the middle cerebral arterys in the experimental group of animals (4D-HBOT) and the control group (CTRL) didn't reveal statistically significant difference. The addition of acetylcholine (endothelium-dependent vasodilation) and SNP (endothelium-independent vasodilation) didn't show a statistically significant difference in flow-induced dilation in 4D-HBOT compared to CTRL. The addition of PSS Ca-free also didn't show statistically significant difference in flow-induced dilation between CTRL and 4D-HBOT. Conclusion: Intermittent hyperbaric oxygenation doesn’t change the response of isolated resistant vessels in flow-induced dilation in healthy man SD rats, nor it affect the response to acetylcholine or SNP, that is endothelium-dependent or independent dilation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:399682
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-14 08:31:54