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master's thesis
Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora
2021. urn:nbn:hr:152:089889
Alpeza, Andrea
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Alpeza, A. (2021). Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:089889

Alpeza, Andrea. "Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:089889

Alpeza, Andrea. "Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:089889

Alpeza, A. (2021). 'Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 18 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:089889

Alpeza A. Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 December 18] Available at: https://urn.nsk.hr/urn:nbn:hr:152:089889

A. Alpeza, "Utjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:089889

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Metadata
TitleUtjecaj visokoslane prehrane na genski izražaj antioksidativnih enzima u mikro- i makrocirkulaciji te bubrezima mladih Spraque-Dawle štakora
Title (english)Influence of high salt diet on antioxidative enzymes gene expression in micro-, macrocirculation and kidneys of young Spraque-Dawley rats
AuthorAndrea Alpeza
MentorAnita Matić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberAnita Matić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
CILJ ISTRAŽIVANJA: Ispitati relativan izražaj važnih antioksidativnih enzima (Cu/Zn SOD, Mn SOD, EC-SOD, GPx1 i GPx4) u mikro-, makrocirkulaciji te bubrezima pod utjecajem visokoslane prehrane. NACRT STUDIJE: Kontrolirani pokus na animalnom modelu MATERIJALI I METODE: Sprague-Dawley muški štakori starosti 3 tjedna podijeljeni su u 4 skupine. Dvije skupine štakora konzumirale su niskoslanu prehranu koja sadrži 0,4 % NaCla u svom sastavu (kroz 7 ili 30 dana), dok su druge dvije skupine konzumirale visokoslanu prehranu koja sadrži 4 % NaCl-a (kroz 7 ili 30 dana). Nakon završenog dijetnog protokola, životinje su žrtvovane dekapitacijom te su prikupljeni uzorci aorti, krvnih žila mozga te bubrega iz kojih se rtPCR metodom odredio genski izražaj ciljnih antioksidativnih enzima. REZULTATI: Visokoslana prehrana uzrokovala je značajnu promjenu Mn SOD antioksidativnog gena u aortama te krvnim žilama mozga. 30 dana visokog unosa soli dovelo je do značajno većeg izražaja Mn SOD u odnosu na akutni (7 dana) protokol. Također, 7 dana visokoslane ishrane značajno je snizio izražaj istog gena u odnosu na niskoslanu skupinu. Ostali antioksidativni enzimi nisu se značajno mijenjali između ispitivanih skupina u svim uzorcima. ZAKLJUČAK: Mlađi štakori bolje kompenziraju štetne utjecaje visokoslane prehrane te je potreban duži period takve ishrane kako bi se pokazali štetni učinci. Mn SOD ima najznačajniju ulogu u promjeni antioksidativnog statusa. Kod dužeg perioda visokoslane prehrane dolazi do aktivacije kompenzacijskog mehanizma i regulacije oksidativnog stresa povećanjem izražaja Mn SOD.
Abstract (english)
OBJECTIVES: To examine the relative expression of important antioxidant enzymes (Cu/Zn SOD, Mn SOD, EC-SOD, and GPx1 GPx4) in micro, macrocirculation and kidneys under the influence of high salt diet. STUDY DESIGN: Controlled experiment in animal model MATERIALS AND METHODS: Sprague-Dawley male rats at 3 weeks of age were divided into 4 groups. Two groups of rats consumed a low-salt diet containing 0,4 % NaCl in their composition (for 7 or 30 days) and the other two groups consumed a high-salt diet containing 4 % NaCl (for 7 or 30 days). After completion of the dietary protocol, the animals were sacrificed by decapitation. From the samples of aorta, blood vessels of the brain and kidneys, the gene expression of target antioxidant enzymes was determined by real-time PCR. RESULTS: A high salt diet caused a significant change in the Mn SOD antioxidant gene in the aorta and blood vessels of the brain. 30 days of high salt intake lead to significantly higher expression of Mn SOD compared to the acute (7 days) protocol. Also, 7 days of a high-salt diet significantly lowered the expression of the same gene relative to the low-salt group. Other antioxidant enzymes did not change significantly between test groups in all samples. CONCLUSION: Younger rats compensate harmful effects of a high-salt diet better and it would take a longer period of such diet to show the harmful effects. Mn SOD has the most significant role in changing the antioxidant status. A longer period of high-salt diet leads to activation of the compensatory mechanism and regulation of oxidative stress by increasing expression of Mn SOD.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:089889
Study programmeTitle: University Graduate Studies
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-18 08:41:00