prikaz prve stranice dokumenta Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola
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master's thesis
Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola
2021. urn:nbn:hr:152:591880
Horvat, Helena
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Horvat, H. (2021). Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:591880

Horvat, Helena. "Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:591880

Horvat, Helena. "Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:591880

Horvat, H. (2021). 'Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 16 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:591880

Horvat H. Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 December 16] Available at: https://urn.nsk.hr/urn:nbn:hr:152:591880

H. Horvat, "Učinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:591880

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TitleUčinci hrane bogate ugljikohidratima i mastima te primjene antidijabetičkog tretmana na vazorelaksaciju u aortama kod pretilih i predijabetičkih odraslih štakora oba spola
Title (english)The effects of carbohydrate and fat-rich food and use of antidiabetic treatment on aortic vasorelaxation in obese pre-diabetic adult rats of both sexes
AuthorHelena Horvat
MentorAnita Matić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberAnita Matić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract
Cilj istraživanja: Odrediti razlike u razini relaksacije inducirane ACh-om te ispitati ulogu antidijabetičkih lijekova kod Sprague-Dawley štakora oba spola hranjenih hranom bogatom mastima i ugljikohidratima. Nacrt studije: Eksperimentalna studija Ispitanici i metode: U istraživanju su korišteni Sprague-Dawley štakori oba spola u starosti od 10 mjeseci podijeljeni u 4 skupine: 1.) Kontrolna skupina hranjena standardnom prehranom, 2.) HSHFD skupina hranjena prehranom bogatom ugljikohidratima i mastima te 3.) i 4.) HSHFD + liraglutid/metformin skupine koje su primale antidijabetički tretman. Nakon završenog dijetnog protokola životinje su izvagane, anestezirane, dekapitirane te im je izolirana torakalna aorta za ispitivanje razine ACh-om potaknute vazorelaksacije u odsutnosti i prisutnosti i L-NAME-a ili se utvrđivala mjera nastalog oksidativnog stresa pomoću TEMPOL-a. Rezultati: Liraglutid dovodi do značajnog snižavanja tjelesne mase kod oba spola. ACh-om posredovana vazorelaksacija je smanjena kod ženki u HSHFD + METFORMIN skupini u usporedbi s kontrolom. Kontrolna skupina muških štakora pokazala je značajno smanjenu bazalnu vrijednost ACh-om uzrokovane relaksacije u usporedbi sa svim ostalim skupinama muških štakora, dok su skupine s antidijabetikom imale bolji odgovor u odnosu na skupinu HSHFD. Usporedbom između spolova pokazalo se kako muški kontrolni štakori imaju smanjenu vazorelaksaciju u odnosu na kontrolnu žensku skupinu. Kod svih ispitanih skupina prisutnost L-NAME-a značajno je smanjila odgovor na ACh, dok TEMPOL ima značajan utjecaj jedino na žensku kontrolnu skupinu. Zaključak: Rezultati sugeriraju da bolji oksidacijski status ženske skupini dovodi do manjih promjena u vazorelaksaciji. Stariji muški štakori imaju izraženije oštećenje vazorelaksacije od starijih ženskih štakora.
Abstract (english)
Objectives: To evaluate the level of relaxation induced by acetylcholine and to examine the role of antidiabetic drugs in Sprague-Dawley rats of both sexes fed food rich with fats and carbohydrates. Study design: Experimental research. Material and Methods: In the study, Sprague-Dawley rats of both sexes were divided into 4 groups at 10 months of age: Control group which was fed a standard diet, HSHFD group which was fed a diet rich in carbohydrates and fats, and HSHFD + metformin and HSHFD + liraglutide groups which, after 4 months of HSHFD diet, received antidiabetic treatment for 14 weeks. After completion of the dietary protocol, the animals were weighed, anesthetized, decapitated, and their thoracic aorta was isolated, which was used to examine the level of acetylcholine-induced vasorelaxation in the absence and presence of NO synthase inhibitor (L-NAME) or to measure the level of oxidative stress with TEMPOL. Results: Liraglutide leads to a significant reduction in body weight in both sexes. Acetylcholine-mediated vasorelaxation was reduced in the female HSHFD + METFORMIN group compared to the control. Also, the male control group showed a reduction in baseline acetylcholine-mediated vasorelaxation compared to all other male groups, while the groups with antidiabetic treatment had a better response compared to the HSHFD group. Comparison between the sexes showed that male control rats had reduced vasorelaxation compared to the female control group. In all examined groups of both sexes, the presence of L-NAME significantly reduced the response to acetylcholine, while TEMPOL had a significant effect only on the female control group. Conclusion: Present results suggest that minor changes in vasorelaxation are in the female group becouse of better oxidative status, and also that aged male rats have more prominent vascular relaxation impairment than female.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:591880
Study programmeTitle: University Graduate Studies
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-12-08 11:39:03