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master's thesis
Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika
2021. urn:nbn:hr:152:751095
Janić, Ana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Janić, A. (2021). Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:751095

Janić, Ana. "Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:751095

Janić, Ana. "Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:751095

Janić, A. (2021). 'Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:751095

Janić A. Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:751095

A. Janić, "Utjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:751095

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TitleUtjecaj kratkotrajnog velikog unosa soli na serumsku razinu proupalnih i protuupalnih citokina u zdravih mladih ispitanika
Title (english)The effect of short-term high-salt diet on serum concentration of pro- and anti-inflammatory cytokines in healthy individuals
AuthorAna Janić
MentorAna Stupin (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberAna Stupin (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-07-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
Cilj istraživanja: Cilj istraživanja bio je ispitati učinak kratkotrajne dijete s velikim udjelom kuhinjske soli (HS) na serumsku razinu proupalnih ( IFN-γ, TNF-α, IL-6, IL-17A, IL-23, IL- 9) i protuupalnih (imunomodulatornih) citokina ( IL-10, IL-21, IL-22) u zdravih mladih ispitanika oba spola korištenjem Procarta PlexMultiplex Immunoassay testova te metode temeljene na tehnologiji LuminexxMAP (engl. multi-analyte profiling). Ustroj studije: Nerandomizirani kontrolirani klinički pokus. Ispitanici i metode: U istraživanju je sudjelovalo 24 mladih zdravih ispitanika (12 žena i 12 muškaraca) starosne dobi 18 - 30 godina. 14 dana su ispitanici bili uključeni u dijetni protokol koji je obuhvaćao 7 dana dijete s malim udjelom soli (LS) (~ 3,5 g soli/dan) i 7 dana dijete s velikim udjelom kuhinjske soli (HS) (~ 14 g soli/dan). Iz skupljenih uzoraka venske krvi mjerila se serumska koncentracija proupalnih i protuupalnih citokina korištenjem ProcartaPlex Multiplex Immunoassay testova te metode temeljene na tehnologiji LuminexxMAP (engl. multi – analyte profiling). Rezultati: Sedam dana dijete s velikim udjelom soli uzrokovalo je povećanje serumske koncentracije proupalnih citokina IFN-γ, IL-17A, IL-9, i protuupalnog citokina IL-10 u usporedbi s mjerenjem prije HS dijete (LS). Serumska koncentracija proupalnih citokina TNF-α, IL-6 i IL-23, kao i protuupalnih citokina IL-21 i IL-22 nije se značajno promijenila nakon 7 dana HS dijete u usporedbi s mjerenjem prije HS dijete (LS). Zaključak: Sedmodnevan povećan unos kuhinjske soli uzrokuje povećanje serumske koncentracije proupalnih citokina IFN-γ, IL-17A i IL-9, ali i protuupalnog citokina IL-10 kod zdravih mladih ispitanika neovisno o promjenama arterijskog krvnog tlaka.
Abstract (english)
Study aim: The aim of this study was to examine the effect of short-term diet with high salt content (HS) on serum levels of pro-inflammatory (IFN-γ, TNF-α, IL-6, IL-17A, IL- 23, IL-9) and anti-inflammatory cytokines (IL-10, IL-21, IL-22) in healthy young subjects of both sexes, using ProcartaPlex Multiplex Immunoassay tests and methods based on LuminexxMAP (multi-analyte profiling) technology. Structure of the study: Non-randomized controlled clinical trial Participants and methods: The study involved 24 young health ysubjects (12 women and 12 men), aged 18 - 30 years. For 14 days, subjects were included in a dietary protocol, which included a 7-day low-salt diet (LS) (~ 3.5 g salt / day) and a 7-day high-salt (HS) diet (~ 14 g salt/day). Serum concentrations of pro- and anti-inflammatory cytokines were measured from collected venous blood samples using ProcartaPlex Multiplex Immunoassay tests and methods based on LuminexxMAP (multi-analyte profiling) technology. Results: The seven-day HS diet significantly increased serum level of proinflammatory cytokines IFN-γ, IL-17A, IL-9, and anti-inflammatory cytokine IL–10 compared to the measurement before HS diet (LS). Serum level of proinflammatory cytokines TNF-α, IL-6 i IL-23, and anti-inflammatory cytokine IL-21 i IL-22 was not significantly changed following 7-day HS diet compared to the measurement before HS diet (LS). Conclusion: One week of a high-salt diet caused a significant increase in serum levels of proinflammatory (IFN-γ, IL-17A, IL-9) and anti-inflammatory (IL-10) cytokines in healthy individuals, independently of blood pressure changes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:751095
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-12-21 09:29:19