OBJECTIVES: The aim of this study was to estimate the survival rate of human epithelial gastric cells (AGS) and to measure the amount of cellular reduced glutathione at different concentrations during 24 hours of amiodarone exposure, using microphotography show changes in the morphology of amiodarone-treated cells, as well as the formation and distribution of lipid droplets by “Oil red O” staining and compare the IC50 of amiodarone by MTT, GSH measurement and “Oil red O” staining. STUDY DESIGN: The study was conducted as a randomized controlled trial. MATERIALS AND METHODS: The human gastric cell line (AGS) was grown in RPMI-1640 medium with 10 % FBS and 100 μg/ml antibiotics/antifungals, at 37 °C, in humid ambient air with 5 % CO2. Cells were grown in 96-well plates for MTT and in 6-well plates for GSH and Oil Red O staining. They were treated with amiodarone at concentrations of 30 μM and 15 μM. Changes in cell vitality, GSH levels, and the appearance of lipid droplets in treated cells compared to controls were recorded by trypan blue, MTT assay, GSH method, and Oil Red O staining. RESULTS: The action of amiodarone at different concentrations led to a dose-dependent decrease in the number of AGS cells in cell culture. Treatment of AGS cells with different concentrations of amiodarone leads to an increase in the number and size of lipid droplets in the cytosol of AGS cells. CONCLUSION: By comparing MTT, GSH and ORO methods in proving the cytotoxicity of amiodarone at concentrations of 30 μM and 15 μM; MTT and ORO showed dose-dependent reduction in cell number, while GSH did not demonstrate the occurrence of oxidative stress. Oil Red O has proven the lipogenic effect of amiodarone.