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master's thesis
Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora
2021. urn:nbn:hr:152:934892
Turza, Lara
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Turza, L. (2021). Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:934892

Turza, Lara. "Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:934892

Turza, Lara. "Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:934892

Turza, L. (2021). 'Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 21 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:934892

Turza L. Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 November 21] Available at: https://urn.nsk.hr/urn:nbn:hr:152:934892

L. Turza, "Utjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:934892

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TitleUtjecaj intermitentne hiperbarične oksigenacije na ulogu ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora
Title (english)Influence of intermittent hyperbaric oxygenation on the role of ion channels in vascular reactivity mechanisms in Sprague-Dawley rats
AuthorLara Turza
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Odrediti djelovanje intermitentne hiperbarične oksigenacije na aktivnost ionskih kanala (BKCa i KATP) kod Sprague-Dawley štakora, metodom mjerenja protokom potaknute dilatacije. Ustroj studije: Eksperimentalna studija na pokusnim laboratorijskim životinjama. Materijali i metode: U svrhu provedbe istraživanja muški SD štakori podijeljeni su u dvije skupine: CTRL skupina – zdravi, netretirani štakori (N=12) i 4D – HBOT skupina – štakori izloženi intermitentnoj hiperbaričnoj oksigenaciji (N=6). Štakori su HBO₂ bili izloženi tijekom četiri uzastopna dana (100 %-tni kisik, 2 h na 2,0 atm). Peti dan, bez izlaganja djelovanju terapije, štakori su izvagani, anestezirani, a potom je uslijedila dekapitacija i izolacija središnje moždane arterije – model na kojem se određivala protokom potaknuta dilatacija bazalno i u prisutnosti antagonista ionskih kanala (BKCa antagonist i KATP antagonist), pri različitim gradijentima tlaka. Rezultati: Nije utvrđena statistički značajna razlika u odgovoru na protokom potaknutu dilataciju bazalno između CTRL i 4D – HBOT skupine štakora. U objema ispitivanim skupinama životinja, FID središnje moždane arterij u prisutnosti antagonista ionskih kanala značajno je smanjena u odnosu na bazični odgovor gotovo pri svim ispitivanim gradijentima tlaka. Zaključak: Intermitentna hiperbarična oksigenacija nema značajan utjecaj na protokom potaknutu dilataciju kod SD štakora. Ionski kanali (BKCa i KATP) imaju važnu ulogu u bazalnom mehanizmu protokom potaknute dilatacije ali i nakon izlaganja intermitentnoj HBO2, što je potvrđeno značajnim smanjenjem FID u prisutnosti antagonista ovih kanala u odnosu na bazični odgovor središnje moždane arterije u obje skupine životinja.
Abstract (english)
Objective: The main goal of this study was to determine the influence of intermittent hyperbaric oxygenation on ion channels (BKCa i KATP) activity in Sprague-Dawley rats, by flow-induced dilation (FID) measurement. Study design: Experimental study on laboratory animals – Sprague-Dawley rats. Materials and methods: Male SD rats were divided into two groups: CTRL group – control group of healthy, untreated rats (N = 12) and 4D - HBOT group - rats exposed to intermittent hyperbaric oxygenation (N = 6). Rats were exposed to HBO₂ for four days (100% oxygen, 2 h at 2,0 atm). Without exposure to a treatment, the rats were weighed and anesthetized on the fifth day. This was followed by decapitation and isolation of the middle cerebral artery - a model on which flow-induced dilation was determined – basal and in the presence of ion channel antagonists (BKCa antagonist i KATP antagonist), at different pressure gradients. Results: No statistically significant difference in the basal response to FID between CTRL and 4D - HBOT group was found. In the presence of ion channel antagonists FID was significantly reduced in both groups of animals at each pressure gradients. Conclusion: Intermittent hyperbaric oxygenation has no significant influence on flow-induced dilation in SD rats. Ion channels (BKCa and KATP) play an important role in the mechanisms of flow-induced dilatation as evidenced by a significant decrease of FID in the presence of channels antagonists relative to the middle cerebral artery basal response in both groups of animals.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:934892
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-03-06 12:24:20