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undergraduate thesis
Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke
2022. urn:nbn:hr:152:063140
Jagodić, Matea
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Jagodić, M. (2022). Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:063140

Jagodić, Matea. "Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:063140

Jagodić, Matea. "Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:063140

Jagodić, M. (2022). 'Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:063140

Jagodić M. Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:063140

M. Jagodić, "Zadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:063140

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Metadata
TitleZadržavanje magnetskih čestica u sferoidima tumorskih stanica dojke
Title (english)Retention of magnetic particles in the spheroids of breast tumor cells
AuthorMatea Jagodić
MentorBarbara Viljetić (mentor)
Committee memberKatarina Mišković Špoljarić (predsjednik povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-07-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Biochemistry
Abstract
Ciljevi: Korištenjem metode magnetske levitacije cilj je uzgojiti sferoide tumora dojke različite starosti, odnosno 7, 14 i 21 dan. Zatim odrediti količinu magnetskih čestica u sferoidima u različitim vremenskim razdobljima, odnosno nakon 7, 14 i 21 dan. Nacrt studije: In vitro studija-kontrolirani pokus. Materijali i metode: 3D kultura stanica dobivena je korištenjem metode magnetske levitacije. Stanicama se dodaju magnetske čestice i uz pomoć vanjskog magnetskog polja formiraju se sferoidi kojima se lako manipulira. Zadržavanje magnetskih čestica u sferoidima stanica tumora dojke MCF-7 praćeno je svjetlosnim mikroskopom na koji je montirana kamera. Prethodno su sferoidi uklopljeni u medij za kriostatsko rezanje, rezani i bojeni. Fotografije su analizirane u programu Fiji, a statistika je napravljena u programu R. Rezultati: Analiziranjem količine magnetskih čestica u sferoidima stanica tumora dojke može se uočiti da je najviše čestica vidljivo u 1. tjednu, te da se njihov broj s vremenom smanjuje. Dobivena je statistički značajna razlika između 1. i 3. tjedna. Zaključak: Količina magnetskih čestica s vremenom se smanjuje. Broj magnetskih čestica značajno je manji 3. tjedan u odnosu na 1. tjedan.
Abstract (english)
Objectives: To grow breast cancer spheroids of different ages, i.e., 7, 14, and 21 days, using the method of magnetic levitation. To determine the number of magnetic particles in the spheroids at different time periods, i.e., after 7, 14, and 21 days. Study Design: In vitro study-controlled experiment Material and methods: 3D cell culture was obtained by the magnetic levitation method. Magnetic particles were added to the cells and with the help an external magnetic field spheroids were formed. The retention of magnetic particles in MCF -7 breast cancer cells spheroids were monitored whit an optical microscope mounted on a camera. Previously, the spheroids were embedded in a medium for cryostat cutting, cut and stained. Photos were analyzed in Fiji and statistics is performed in R. Results: When monitoring the retention of magnetic particles in breast cancer cells spheroids, the most particles were visible in the 1st week. By the 3rd week, a decrease in the number of particles was observed. A statistically significant difference was obtained between the 1st and 3rd week. Conclusion: The number of magnetic particles decreases with time. It is significantly lower in the 3rd week compared to the 1st week.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:063140
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-06-01 11:43:41