Objectives: The objectives of this study were to examine whether there are differences in the dimensions, echogenicity and vascularization of benign and malignant axillary lymph nodes. Study design: Cross-sectional research with historical data. Participants and methods: A total of 25 respondents of all age groups participated in the study. Of the 25 respondents, 18 are women and 7 are men. The included respondents were examined in the period from December 2013 until May 2015. During the ultrasound examination, if it is determined that the lymph node is morphologically altered or is larger than expected, this is an indication for ultrasound-controlled cytological puncture. The analysis of clinical data will be collected from the medical documentation of the Clinical Institute for Diagnostic and Interventional Radiology of the Clinical Hospital Center Osijek. Results: The results of the examined ultrasound features showed, although not significant, the difference between benign and malignant lymph nodes. Malignant lymph nodes proven by cytological findings were slightly larger than benign lymph nodes. They were also heteroechoic and hypoechoic structures in contrast to hyperechoic benign lymph nodes. Vascularization showed that all benign lymph nodes were correct vascularizations, while malignant lymph nodes were of all vascular categories, but the majority (54%) were irregular vascularizations. Conclusion: Based on the results of the research, we concluded and confirmed the importance of ultrasound diagnostics and monitoring of lymph node characteristics and the importance of cytological puncture under ultrasound control. We also concluded that the results of ultrasound features of lymph nodes may vary and are not always a reliable presentation. We have proven that proper determination and monitoring of ultrasound features can greatly help early cytological diagnosis of malignant changes.