Aim: The main goal of the doctoral thesis is to examine emotional regulation and control (ERC), as well as types of stressors in the workplace of health workers, i.e. a medical institution, and to determine the correlation and impact of stressors on the ability of ERC The aim is to determine the contribution of the ERC ability to the development of stress in healthcare workers. Design: cross-sectional survey Participants and methods: The research was based on the participation of 203 health workers employed at the University Hospital Center Osijek in Croatia, in inpatient departments. The research instruments were standardized validated questionnaires, i.e. the Questionnaire on stressors in the workplace of hospital health workers, and the Emotional Regulation and Control Questionnaire. Results: The scale of experienced stress and the ERC scale are related significantly in all the domains, including the total scales, with the exception of the domain Shift work, which is not significantly related to the domain Recollection of emotionally saturated memories, nor the domain Effect of emotions and mood. All the correlations are positive and weak (Rho < 0.5). The total scale of experienced stress is higher if the ERC scale is rated higher, that is, the lower the regulation and control, the more pronounced the experienced stress (Rho = 0.308). Participants who are more exposed to stress in their workplace have significantly less recollection of emotionally saturated memories (P = 0.01) and the overall ERaC scale (P = 0.04). Participants who have a stronger recollection of emotionally saturated memories have a higher chance (odds ratio 1.18; 95% confidence interval 1.07 to 1.30) of experiencing stronger stress. The model is statistically significant in its entirety and explains 11.2% of the variance in the presence of stronger experienced stress, and accurately classifies 72.4% of the cases. The results of the regression analysis clearly show that the recollection of emotionally saturated memories is significant in the prediction of higher quantities of experienced stress among healthcare workers, and as a model, it accounts for 8.8% of the difference in the total experienced stress. Conclusion: The results of this study confirmed the hypothesis that workplace stressors are related to ERC, as well as that they contribute to the development of the ability of ERCin the hospital’s healthcare workers. Healthcare workers who have a better ability of emotion regulation were less influenced by workplace stressors than the employees whose such ability is lower.