Objectives: The aim of the study was to examine the frequency of positive skin test results for hypersensitivity to local anesthetics and to analyze the relationship between the results and severity and characteristics of previous adverse reactions, time interval from reaction to testing, presence of hypersensitivity to other allergens and other drugs, demographic characteristics and existing comorbidities. Study design: The study was conducted as a cross-sectional study. Subjects and methods: The study included patients who were tested for hypersensitivity to local anesthetics in Clinical Pharmacology outpatient clinic at the Clinic for Internal Diseases as a part of University Hospital Center Osijek, in the period from October 2016 to the end of December 2020. The total number of subjects was 73, and data collected from medical records for each patients included demographic dana with comorbidities, data on hypersensitivity to other allergens and other groups of drugs, positive epicutaneous test for benzocaine in the anamnesis, description of previous adverse reaction to local anesthetic, time interval from reaction to testing and outcomes of skin prick tests, intradermal tests and subcutaneous exposure. The collected data were analyzed in relation to the outcome of skin testing. Results: Although the percentage of positive tests is very low (3 %), a higher percentage of positive intradermal tests is recorded compared to the prick tests, with the highest number of positive intradermal tests for artikain + adrenaline. Subcutaneous exposure was negative in all subjects. Adverse reactions to local anesthetics were most often manifested in the form of skin changes. A significant association was observed between a previous adverse reaction to local anesthetics in the form of a skin change and a negative intradermal test for artikain + adrenaline. No significant association of other variables in relation to skin test outcomes was observed. Conclusion: This cross-sectional study confirmed that the frequency of positive skin test results for hypersensitivity to local anesthetics is low, i.e. that true hypersensitivity to local anesthetics is extremely rare.