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undergraduate thesis
Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti
2022. urn:nbn:hr:152:949833
Lukačević, Ivana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Lukačević, I. (2022). Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:949833

Lukačević, Ivana. "Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:949833

Lukačević, Ivana. "Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:949833

Lukačević, I. (2022). 'Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:949833

Lukačević I. Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:152:949833

I. Lukačević, "Uloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:949833

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Metadata
TitleUloga ionskih kanala u protokom-potaknutoj dilataciji kod Sprague- Dawley štakora na visokoslanoj dijeti
Title (english)The role of ion channels in flow-induced dilation in Sprague-Dawley rats on a high-salt diet
AuthorIvana Lukačević
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Odrediti ulogu KATP, KCa2+ i TRPV4 ionskih kanala u protokom potaknutoj dilataciji kod Sprague-Dawley štakora na visokoslanoj dijeti. Nacrt studije: Eksperimentalna studija na izoliranim krvnim žilama. Materijali i metode: Kao model ovom istraživanju poslužile su izolirane središnje moždane arterije muških Sprague-Dawley štakora, starosti 9-11 tjedana, koji su bili podijeljeni u dvije skupine - kontrolnu (CTRL, štakori hranjeni standardnom hranom za glodavce) i visokoslanu (HSD) skupinu (štakori koji su konzumirali hranu s 4% NaCl-a). Na izoliranim žilama, koje su bile postavljene u sustav tlačnog miografa (Myograph Pressure System Model 110P MyoView Version 1.2.0 DMT; Danish Myo Technology) i pričvršćene između dvije staklene pipete, određivala se reaktivnost u odgovoru na stupnjevito povećanje protoka, bazalno te u prisutnosti agonista / antagonista ionskih kanala KATP, KCa te TRPV4. Rezultati: FID je značajno snižen kod visokoslane skupine pri svakom gradijentu tlaka u odnosu na kontrolnu skupinu. Svaki od primijenjenih antagonista doveo je do smanjenog FIDa, pri svakom gradijentu tlaka kod CTRL skupine, dok je u HS skupini FID smanjen samo pri Δ20 mmHg i Δ40 mmHg, dok je pri višem gradijentu tlaka (Δ80 mmHg i Δ100 mmHg) samo antagonist TRPV4 kanala značajno djelovao na smanjenu protokom potaknutu dilataciju. U CTRL skupini, niti jedan od primijenjenih agonista ionskih kanala nije imao značajan učinak na FID, dok je u HS skupini FID značajno obnovljen nakon primjene svakog od pojedinih agonista ionskih kanala. Zaključak: Visokoslana dijeta utječe na mehanizam protokom potaknute dilatacije moduliranjem ionskih kanala.
Abstract (english)
The role of ion channels in flow-induced dilation in Sprague-Dawleyrats on a high-salt diet Aim: The aim of this study is to determine the role of KATP, KCa and TRPV4 ion channels in mechanism of flow-induced dilation of Sprague-Dawley (SD) rats subjected to a high salt (HS) diet. Study design: Experimental study on isolated blood vessels. Materials and methods: The experiment was conducted on model of isolated middle cerebral arteries of male Sprague-Dawley rats, 9-11 weeks old, which were divided into two groups - control (CTRL, rats fed with standard rodent chow) and hight salt (HSD) group (rats fed with 4% NaCl). Isolated vessels, a model on which flow-induced dilatation was determined, were set in the pressure myograph system (Myograph Pressure System Model 110P MyoView Version 1.2.0 DMT; Danish Myo Technology) and cannulated between two glass pipettes, reactivity was determined in response to a stepwise increases in pressure gradients, at the base level and in the pressence of KATP, KCa and TRPV4 ion channel agonists/antagonists. Results: FID was significanty reduced in HS compared with CTRL group at each pressure gradient. Each of applied antagonists reduced FID at each pressure gradient in CTRL group, whereas in HS group impaired FID at Δ20 and Δ40 mmHg, but at high pressure gradient (Δ80 and Δ100 mmHg) only TRPV4 antagonist reduced FID. In CTRL group, each of applied agonists has no effect on FID, whereas in HS group, each of them restored FID at each pressure gradients. Conclusion: Results suggest that HS dietry intake affect the mechanisms of FID by modulating ion channels.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:949833
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
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Created on2024-03-07 13:43:38