prikaz prve stranice dokumenta Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane
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undergraduate thesis
Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane
2022. urn:nbn:hr:152:100113
Bolmanac, Klaudija
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Bolmanac, K. (2022). Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:100113

Bolmanac, Klaudija. "Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:100113

Bolmanac, Klaudija. "Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:100113

Bolmanac, K. (2022). 'Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 11 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:100113

Bolmanac K. Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 December 11] Available at: https://urn.nsk.hr/urn:nbn:hr:152:100113

K. Bolmanac, "Utjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:100113

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TitleUtjecaj angiotenzina II i tempola na promjenu genskog izražaja upalnih biljega u krvnim žilama mozga Spraque-Dawley štakora pod utjecajem visokoslane prehrane
Title (english)The effect of angiotensin II and tempol on inflammatory markers gene expression changes in brain blood vesels of Spraque-Dawley rats under the influence of a high salt diet
AuthorKlaudija Bolmanac
MentorInes Drenjančević (mentor)
Committee memberZrinka Mihaljević (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Ispitati utjecaj visokoslane prehrane te ulogu angiotenzina II i TEMPOLA na genski izražaj biljega upale (IL-1, IL-6, MPC, E-selektina i ICAM-a) u krvnim žilama mozga muških Sprague-Dawley štakora. Nacrt studije: Pokusno (eksperimentalno) istraživanje. Materijali i metode: Muški Sprague-Dawley štakori starosti 9 - 11 tjedana podijeljeni su u 4 skupine. Jedna od skupina je bila podvrgnuta niskoslanoj prehrani te u konzumirale prehranu koja sadrži 0,4 % NaCl-a, te ostale tri skupine koje su konzumirale visokoslanu prehranu koja sadrži 4 % NaCl-a u svom sastavu tijekom 7 dana. Uz prehranu s visokim udjelom soli dvjema skupinama je uveden i dodatni tretman. Jedna je od skupina tijekom cijelog protokola konzumirala vodu u kojoj je bio otopljen TEMPOL u koncentraciji 1 mmol/L, dok je drugoj visokoslanoj skupini 4. dan ugrađena potkožno osmotska minipumpa koja je otpuštala 100ng/kg/min ANG II kroz 3 dana. Nakon završenog protokola životinje su anestezirane, dekapitirane te su im izolirane krvne žile mozga iz kojih je metodom PCR u realnom vremenu određen genski izražaj upalnih biljega (IL-1, IL-6, MPC, E-selektin i ICAM). Rezultati: Analizom genskog izražaja nije utvrđena značajna statistička razlika u vrijednostima između ispitivanih skupina (IL-1 p=0,142; IL-6 p=0,717; MPC p=0,126; E-selektin p=0,348; ICAM p=0,408). Skupina HS+ANG II za biljeg MPC pokazuje tendenciju sniženja genskog izražaja u odnosu na HS skupinu, ali bez dobivene značajnosti. Zaključak: Angiotenzin II i TEMPOL nisu uzrokovali značajnu promjenu genskog izražaja upalnih biljega u odnosu na visokoslanu prehranu. Bilo bi potrebno povećati broj uzoraka ili produžiti vrijeme terapije kako bi se uočile potencijalne razlike.
Abstract (english)
Objectives: To investigate the influence of a high-salt diet and the role of angiotensin II and TEMPOL on the gene expression of inflammation markers (IL-1, IL-6, MPC, E-selectin and ICAM) in the brain blood vessels of male Sprague-Dawley rats. Study design: Experimental research Materials and Methods: Male Sprague-Dawley rats, 9-11 weeks old were divided into 4 groups. One of the groups was subjected to a low-salt diet and consumed a diet containing 0,4% NaCl, and the other three groups who consumed a high-salt diet containing 4% NaCl in its composition for 7 days. In addition to a high-salt diet, additional treatment was introduced for the two groups. One group consumed water in which TEMPOL was dissolved at a concentration of 1 mmol / L throughout the protocol, while the other high-salt group was fitted with a subcutaneous osmotic minipump on day 4 that released 100 ng / kg / min angiotensin II for 3 days. After the protocol was completed, the animals were anesthetized, decapitated, and their brain blood vessels were isolated, from which the gene expression of inflammatory markers (IL-1, IL-6, MPC, E-selectin, and ICAM) was determined by real-time PCR. Results: Analysis of gene expression did not reveal a significant statistical difference between the examined groups (IL-1 p = 0,122; IL-6 p = 0,717; MPC p = 0,126; E-selectin p = 0,348; ICAM p = 0,408). The HS + ANG II group for the MPC marker shows a tendency to decrease gene expression compared to the HS group, but without the obtained significance. Conclusion: Angiotensin II and TEMPOL did not cause a significant change in the genetic expression of inflammatory markers compared to a high-salt diet. It would be necessary to increase the number of samples or extend the time of therapy to notice potential differences.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:100113
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-03-08 06:31:15