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undergraduate thesis
Morfologija ko-kultiviranih sferoida
2022. urn:nbn:hr:152:618443
Kadović, Tin
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kadović, T. (2022). Morfologija ko-kultiviranih sferoida (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:618443

Kadović, Tin. "Morfologija ko-kultiviranih sferoida." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:618443

Kadović, Tin. "Morfologija ko-kultiviranih sferoida." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:618443

Kadović, T. (2022). 'Morfologija ko-kultiviranih sferoida', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 12 May 2024, https://urn.nsk.hr/urn:nbn:hr:152:618443

Kadović T. Morfologija ko-kultiviranih sferoida [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 May 12] Available at: https://urn.nsk.hr/urn:nbn:hr:152:618443

T. Kadović, "Morfologija ko-kultiviranih sferoida", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:618443

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Metadata
TitleMorfologija ko-kultiviranih sferoida
Title (english)Morphology of co-cultivated spheroids
AuthorTin Kadović
MentorTeuta Opačak-Bernardi (mentor)
Committee memberMarijana Leventić (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Uzgojiti sferoide korištenjem metode magnetske levitacija te promatrati i proučavati količinu kolagena koja se stvara u sferoidima nakon jedan,dva i tri tjedna Nacrt studije: In vitro studija – kontrolirani pokus. Metode i materijali: Stanične linije D54 i BJ su inkubirane s magnetskim česticama te inkubirane na 37⁰C, uz 5 % CO₂ i visoku vlažnost zraka. Nakon toga, 5000 stanica svake stanične linije s magnetskim česticama stavljeno je u pločicu s 24 jažice te poklopljeno pločicom za magnetsku levitaciju. U periodu od tri tjedna sferoidi su rezani na kriostatu i bojani metodom po Massonu. Zatim su mikroskopirani i slikani na svjetlosnom mikroskopu s kamerom te su dobivene slike analizirane pomoću programa ImageJ. Rezultati: Količina stvorenog kolagena u sferoidima raste iz tjedna u tjedan. Također dolazi do razvoja izvanstaničnog matriksa. Porast količine kolagena je statistički značajan. Zaključak: Kako sferoid raste i razvija se, tako i stvara sve više kolagena te dolazi do razvoja izvanstaničnog matriksa.
Abstract (english)
Objectives: Grow spheroids using the magnetic levitation method and analyse the quantity of collagen produced during the 3 weeks of growth Study design: In vitro study – controlled experiment. Methods and materials: BJ and D54 cells were incubated with magnetic particles and at 37⁰C with high humidity and 5 % CO₂. After that, 5000 cells of each cell line with magnetic particles were put into a 24- well plate and covered with a magnetic plate. In the period of next three weeks spheroids were cut using a cryostat and then stained with Masson stain. Then, they were examined using a microscope and photographed and the pictures were analysed using the ImageJ programme. Results: The amount of collagen in spheroids increased during the period of three weeks. Spheroids developed extracellular matrix. The increase in the amount of collagen, the difference was statistically important. Conclusion: As the spheroid grows and develops, it creates more collagen and extracellular matrix.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:618443
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-03-08 08:03:58