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undergraduate thesis
Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E
2022. urn:nbn:hr:152:905953
Lagator, Maja
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Lagator, M. (2022). Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:905953

Lagator, Maja. "Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:905953

Lagator, Maja. "Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:905953

Lagator, M. (2022). 'Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:905953

Lagator M. Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:905953

M. Lagator, "Izražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:905953

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TitleIzražaj mRNA antioksidativnih enzima kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E
Title (english)Anitoxidative enzymes' mRNA expression in athletes consuming hen eggs enriched with n-3 polyunsaturated fatty acids, lutein, selenium and vitamin D
AuthorMaja Lagator
MentorInes Drenjančević (mentor)
Committee memberZrinka Mihaljević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
CILJ ISTRAŽIVANJA: Cilj je bio odrediti mRNA izražaj antioksidativnih enzima GPx1, GPx4, SOD2 i CAT kod sportaša koji su konzumirali obična ili obogaćena kokošja jaja. NACRT STUDIJE: Randomizirana, dvostruko slijepa, kontrolirana intervencijska studija (Clinical Trials ID: NCT04564690). ISPITANICI I METODE: Ispitanici su zdravi, mladi sportaši podijeljeni u dvije skupine: kontrolna (N=6) i Nutri4 (N=8). Ispitanici u skupini "Nutri4" konzumirali su kokošja jaja obogaćena n3-PUFA (1026 mg/dnevno), luteinom (616 mg/dnevno), selenom (0,191 mg/dnevno) i vitaminom E (1098 mg/dnevno), a kontrolna grupa je konzumirala obična kokošja jaja (438 mg n-3 PUFA, 110 mg luteina, 0,181 mg selena i 595 mg vitamina E dnevno). Izražaj mRNA Gpx1, Gpx4, SOD2 i CAT određena je PCR-om u stvarnom vremenu (BioRad, CFX96 Real – Time System). Rezultati su normalizirani na GAPDH housekeeping gen i kontrolu i prikazani kao preklopna promjena (2−ΔΔCT). Jednosmjerna ANOVA je provedena za statističku analizu s Tukey post hoc testom; p<0,05 smatra se statistički značajnim. REZULTATI: Konzumacija obogaćenih kokošjih jaja dovela je do značajnog povećanja mRNA izražaja SOD2, dok ostali enzimi nisu imali značajnu razliku u kontrolnoj, eksperimentalnoj niti između dvije skupine. ZAKLJUČAK: Dosadašnji rezultati podupiru hipotezu da funkcionalna hrana može biti prirodni antioksidans.
Abstract (english)
mRNA EXPRESSION OF ANTIOXIDANT ENZYMES IN ATHLETES CONSUMING ENRICHED HEN EGGS OBJECTIVES: The aim of this study was to assess mRNA expression of antioxidant enzymes Gpx1, Gpx4, SOD2 and CAT in athletes who consumed regular hen eggs or enriched eggs. STUDY DESIGN: Randomized, double-blind, controlled interventional study (Clinical Trials ID: NCT04564690). PARTICIPANTS AND METHODS: This study was conducted in young, healthy athletes divided to: control group (N=6) and Nutri4 group (N=8) who consumed 3 hen eggs per day for 3 weeks. Subjects in the "Nutri4" group consumed hen eggs enriched with n3-PUFAs (1026 mg/daily), lutein (616 mg/daily), selenium (0.191 mg/daily) and vitamin E (1098 mg/daily) and control subjects consumed regular hen eggs (438 mg of n-3 PUFAs, 110 mg of lutein, 0.181 mg of selenium and 595 mg of vitamin E daily). mRNA expression of Gpx1, Gpx4, SOD2 and CAT was determined with real time PCR (BioRad, CFX96 Real – Time System). Results were normalized to GAPDH housekeeping gene and control and presented as fold change (2−ΔΔCT). One way ANOVA was conducted for statistical analysis with Tukey post hoc test; p<0.05 was considered statistically significant. RESULTS: Consumption of enriched hen eggs caused a significant increase in SOD2 mRNA expression, while other antioxidative enzymes’ mRNA expression did not exhibit significant difference in control, NUTRI4 or between groups. CONCLUSION: Current results support the hypothesis that functional food has a capacity to act as natural antioxidant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:905953
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-03-12 11:57:07