OBJECTIVES: The objectives of the study were to examine whether there were differences in the use of anxiolytics between healthcare professionals in the hospital and healthcare professionals outside the hospital¸ depending on their workplace, level of education and seniority, as well as the influence of working conditions on self-perception of stress, quality of life and life satisfaction between healthcare professionals in hospital and outpatient conditions. STUDY DESIGN: Cross-sectional research. PARTICIPANTS AND METHODS: The research included 159 healthcare professionals in Slavonski Brod: 96 employees of the General Hospital "Dr. Josip Benčević" and 63 employees of the Health Center and the Institute for Emergency Medicine of Brodsko-Posavina County. Respondents participated in the study by filling out online questionnaires. The questionnaire was voluntary and anonymous and consisted of 53 questions. RESULTS: Statistically significant differences were shown in the perception of stress, which is greater in hospital staff, then in the difference between stressors in the workplace, where hospital staff showed higher values in all categories, but three factors are more significant differences: "Organization of the workplace and financial issues", "Conflicts and communication at work" and "Professional and intellectual requirements". Statistically significant differences exist in the frequency of taking anxiolytics with the help of a psychiatrist. Working conditions have a much greater impact on the perception of stress and life satisfaction in hospital staff, while in outpatient staff only a weak link between the perception of stress and life satisfaction is expressed. CONCLUSION:There is no significant difference in anxiolytic consumption between healthcare professionals in hospital and outpatient conditions.