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undergraduate thesis
Utjecaj sastava i svježine medija na rast stanica
2023. urn:nbn:hr:152:155312
Kovačević, Nikolina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kovačević, N. (2023). Utjecaj sastava i svježine medija na rast stanica (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:155312

Kovačević, Nikolina. "Utjecaj sastava i svježine medija na rast stanica." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:152:155312

Kovačević, Nikolina. "Utjecaj sastava i svježine medija na rast stanica." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:152:155312

Kovačević, N. (2023). 'Utjecaj sastava i svježine medija na rast stanica', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 12 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:155312

Kovačević N. Utjecaj sastava i svježine medija na rast stanica [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2023 [cited 2024 December 12] Available at: https://urn.nsk.hr/urn:nbn:hr:152:155312

N. Kovačević, "Utjecaj sastava i svježine medija na rast stanica", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:152:155312

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TitleUtjecaj sastava i svježine medija na rast stanica
Title (english)Effect of media composition and freshness on cell growth
AuthorNikolina Kovačević
MentorTeuta Opačak-Bernardi (mentor)
Committee memberKatarina Mišković Špoljarić (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2023-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Ciljevi istraživanja su odrediti brzinu rasta stanica u različitim najčešće korišćenim medijima i odrediti utjecaj promjene medija na brzinu rasta. Nacrt studije: In vitro studija – kontrolirani pokus Materijali i metode: Korištene su stanične linije Caco-2 i THP-1 kultivirane u tri različita medija: DMEM, RPMI i OptiMEM. Stanice smo brojali,bojanjem eritrozinom, svaka 24 sata tijelom trajanja istraživanja.Osim brojanja, rast stanica je praćen fotografiranjem kulture u pravilnim vremenskim razmacima. Rezultati: Obje stanične linije najbolje rastu u preporučenom mediju. Brzina rasta u drugim medijima je sporija, ali se stanice u oba zamjenska medija dobro razvijaju. THP-1 stanice su osjetljivije na promejnu medija te je primjećena značajna razlika u broju stanica u usporedbi sa zamjenskim medijima, dok je razlika kod Caco-2 stanica vidljiva tek nakon dužeg uzgoja. Zaključak: Iz provdenog istraživanja i dobivenih rezultata možemo zaključiti da promjena medija utječe na brzinu rasta stanica ali stanice mogu rasti u zamjenskim medijima neko vrijeme. Korištenje preporučenog medija osigurava najbrži rast stanica u kulturi.
Abstract (english)
Effect of media composition and freshness on cell growth Objectives: The objectives of the research are to determine the growth rate of cells in various commonly used media and to determine the influence of media change on the growth rate. Study Design: In vitro study - controlled experiment Material and Methods: The used cell lines Caco-2 and THP-1 were cultured in three different media: DMEM, RPMI and OptiMEM. We counted the cells, by staining them with erythrosin, every 24 hours for the duration of the research. In addition to counting, cell growth was monitored by photographing the culture at regular intervals. Results: Both cell lines grow best in the recommended medium. The growth rate in the other media is slower, but the cells grow well in both replacement media. THP-1 cells are more sensitive to media change, and a significant difference in the number of cells was observed compared to replacement media, while the difference in CaCO-2 cells is visible only after longer cultivation. Conclusion: From the conducted research and the obtained results, we can conclude that the change of media affects the speed of cell growth, but cells can grow in replacement media for some time. Using the recommended medium ensures the fastest growth of cells in culture.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:155312
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-05-09 06:42:50