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master's thesis
Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora
2024. urn:nbn:hr:152:771949
Kovač, Lora
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kovač, L. (2024). Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:771949

Kovač, Lora. "Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:771949

Kovač, Lora. "Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:771949

Kovač, L. (2024). 'Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:771949

Kovač L. Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2024 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:152:771949

L. Kovač, "Utjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:152:771949

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Metadata
TitleUtjecaj oralnog nadomjestka karnozina tijekom visokoslane prehrane na izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora
Title (english)Effect of oral carnosine supplementation during a high-salt diet on gene expression of antioxidant enzymes in blood vessels of Sprague- Dawley rats
AuthorLora Kovač
MentorZrinka Mihaljević (mentor)
Committee memberAna Stupin (predsjednik povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2024-06-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Cilj diplomskog rada je odrediti učinak nadomjestka karnozina na izražaj gena antioksidativnih enzima u uzorcima aorte Sprague-Dawley štakora izloženih standardnoj i visokoslanoj prehrani. Nacrt studije: Eksperimentalna studija na pohranjenim aortama Sprague-Dawley štakora. Materijal i metode: Zdravi Sprague-Dawley štakori oba spola (starosti 8-10 tjedana) nasumično su podijeljeni u četiri skupine: kontrolna skupina (CTRL, standarna prehrana s 0,4 % NaCl), skupina na visokoslanoj prehrani (HSD, 4 % NaCl), skupina koja je konzumirala karnozin (CTRL + CAR, 150 mg/kg) te skupina na visokoslanoj prehrani koja je konzumirala karnozin (HSD + CAR). Nakon jednotjednog protokola, štakori su anestezirani i izolirani su uzorci aorte. Nakon ekstrakcije RNA, određen je relativni izražaj SOD2, SOD3, GPx1, GPx4 i CAT uz korištenje βaktina kao housekeeping gena. Rezultati: Razina genskog izražaja SOD2, GPx1, i GPx4 povišena je u HSD + CAR skupini. Izražaj SOD2 također je povišen u CTRL + CAR skupini. Nije uočena značajna promjena izražaja SOD3 i CAT. Buduća istraživanja mogu se usmjeriti na određivanje izražaja proteina te aktivnosti enzima kako bi se dodatno razjasnila uloga karnozina. Zaključak: Primjena karnozina dovela je do povećanog izražaja gena antioksidativnih enzima tijekom visokoslane prehrane.
Abstract (english)
Objectives: The study aimed to determine the impact of carnosine supplementation on relative expression of antioxidant enzyme genes in samples of rat aortas during a normal diet and a highsalt diet. Study design: Experimental animal study using isolated blood vessels of Sprague-Dawley rats. Material and methods: Healthy, male and female Sprague-Dawley rats (aged 8 - 10 weeks) were randomly divided into four groups: the control group (CTRL, standard 0,4 % NaCl diet), high-salt diet group (HSD, 4 % NaCl), group that was administered carnosine (CTRL + CAR, 150 mg/kg oral carnosine) and the high-salt diet group that was simultaneously given carnosine (HSD + CAR). After the one week long protocol, the rats were anesthetized, and samples of the aorta were taken. After RNA extraction, relative gene expressions of SOD2, SOD3, GPx1, GPx4 and CAT were measured using RTqPCR, with β-actin as the housekeeping gene. Results: In the HSD + CAR group, levels of SOD2, GPx1 and GPx4 expression were increased. SOD2 was also elevated in the carnosine supplemented group that was fed a normal diet. No notable change was recorded in SOD3 and CAT expression. Future research can be directed towards determining protein expression and enzyme activity to further clarify the role of carnosine. Conclusion: Administration of carnosine led to an increase in relative expression of antioxidant enzyme genes during a high-salt diet.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:771949
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-11 11:53:48