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master's thesis
Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša
2024. urn:nbn:hr:152:956946
Križaić, Karolina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Križaić, K. (2024). Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:956946

Križaić, Karolina. "Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:956946

Križaić, Karolina. "Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:956946

Križaić, K. (2024). 'Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 01 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:956946

Križaić K. Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2024 [cited 2024 November 01] Available at: https://urn.nsk.hr/urn:nbn:hr:152:956946

K. Križaić, "Utjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:152:956946

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Metadata
TitleUtjecaj enzimskog uklanjanja fosfolipida na prikaz gangliozida u mozgu miša
Title (english)The influence of enzymatic removal of phospholipids on the representation of gangliosides in mouse brain
AuthorKarolina Križaić
MentorMarija Heffer (mentor)
Committee memberVedrana Ivić (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2024-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Cilj ovog istraživanja je utvrditi utjecaj fosfolipaze A1 (PLA1) na imunohistokemijsku vizualizaciju gangliozida u malom mozgu miša. Nacrt studije: Eksperimentalna studija, ustrojena kao istraživanje parova, provedena na tkivu mozga miša. Materijali i metode: Istraživanje je provedeno na tkivu maloga mozga prikupljenog od miševa divljeg tipa (soj B6/129S). Ukupno su žrtvovana tri miša muškog spola starosti 7. mjeseci. Fiksirani uzorci malog mozga podijeljeni su u dvije skupine, gdje je eksperimentalna skupina bila izložena tretmanu fosfolipaze A1 (PLA1). Imunohistokemija slobodno plutajućih rezova tkiva tretiranih ili ne tretiranih s PLA1 napravljena je uz pomoć visokospecifičnih monoklonalnih IgG protutijela za GM1, GD1a, GD1b i GT1b gangliozide. Rezultati su kvantificirani u Fiji programu. Rezultati: Kod sva četiri istraživana gangliozida, nakon tretmana s PLA1, promijenjen je obrazac obojenja slojeva malog mozga. Intenzitet obojenja GM1 bio je veći kod tretiranih uzoraka, ali je rezultirao gubitkom obojenja GD1a. Isti je tretman doveo do sniženja signala GD1b i GT1b u molekularnom sloju i povišenja u zrnastom sloju. Zaključak: Tretman fiksiranih rezova tkiva s PLA1 značajno je promijenio količinu i distribuciju signala za sva četiri istraživana gangliozida što bi moglo biti povezano s razlikama u distribuciji ovih gangliozida u lipidnim domenama membrane.
Abstract (english)
Objectives: The aim of this research is to determine the influence of phospholipase A1 (PLA1) on the immunohistochemical visualization of gangliosides in the mouse cerebellum. Study design: An experimental study, designed as a pair study, conducted on mouse brain tissue. Materials and methods: The research was conducted on cerebellar tissue collected from wildtype mice (strain B6/129S). A total of three 7-month-old male mice were sacrificed. Fixed cerebellum samples were divided into two groups, where the experimental group was exposed to phospholipase A1 (PLA1) treatment. Immunohistochemistry of free-floating tissue sections treated or not treated with PLA1 was performed using highly specific monoclonal IgG antibodies to GM1, GD1a, GD1b and GT1b ganglioside. The results are quantified in the Fiji program. Results: With all four investigated gangliosides, after treatment with PLA1, the staining pattern of the layers of the cerebellum changed. GM1 staining intensity was higher in treated samples but resulted in loss of GD1a staining. The same treatment led to a decrease in GD1b and GT1b signals in the molecular layer and an increase in the granular layer. Conclusion: Treatment of fixed tissue sections with PLA1 significantly changed the amount and distribution of signals for all four investigated gangliosides, which could be related to differences in the distribution of these gangliosides in the lipid domains of the membrane.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:956946
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-17 13:39:50