OBJECTIVES: This paper aims to compare the qualitative immunochemical method with the new quantitative method for determining fecal hemoglobin and to examine whether there is a statistically significant difference in the results. STUDY DESIGN: A cross-sectional study. PARTICIPANTS AND METHODS: This study included 24 random stool samples from patients from the Department of Gastroenterology and Hepatology of the University Hospital Center Osijek (KBC), and were analyzed using the qualitative CoproHemoGnost method and the quantitative method on the OC-SENSOR Ceres analyzer. The normality of the distribution of numerical variables was tested by the Shapiro-Wilk test, and the data were described by the median and boundaries of the interquartile range. The Kappa test was used to test the agreement between the two methods. RESULTS: A good agreement was observed between the two methods for determining fecal hemoglobin using the Kappa test with a 95% confidence interval ( = 0.65). There were also 4 results that were inconsistent when compared. Hb concentrations are relatively evenly distributed and there are no large extremes. CONCLUSION: A comparison of the qualitative and quantitative FIT method on an automatic analyzer did not reveal a statistically significant difference in the results. The methods are well matched and complemented, but the results of the qualitative FIT method should be confirmed by a quantitative method that has the ability to give numerical concentrations.