AIM: The aim of the study was to examine the difference in the extent of eye movement and non-motor symptoms in patients with cervical dystonia before and after botulinum toxin therapy, whereby a significant difference would speak in favor of the central effect of the drug. STUDY DESIGN: Prospective study of pairs in comparison with a control group. RESPONDENTS AND METHODS: A total of 60 respondents participated in the study (30 patients and 30 healthy controls). Instruments that were used to examine the clinical picture and non-motor symptoms in patients with cervical dystonia were TSUI and TWSTRS rating scales for examining the severity of the clinical picture, pain and disability, while validated questionnaires for the assessment of psychiatric disorders BDI-II ( Beck Depression Inventory), BAI (Beck Anxiety Inventory), autonomic disorders COMPASS-31 (Composite Autonomic Symptom Score) and cognitive status ACER (Adenbrooke's Cognitive Examination) were used in all respondents. Eye movements were analyzed using a Tobii TX300 eye-tracker, while the software used in the research was the one developed at the Faculty of Computer Science and Informatics (artificial intelligence) of the University of Ljubljana. In patients with cervical dystonia, eye movements, motor and non-motor symptoms were compared before and after botulinum toxin therapy, while the difference in eye movements and non-motor symptoms was studied between the patients and the control group. RESULTS: There is a clear difference in eye movements in patients with cervical dystonia compared to healthy controls. After the administration of botulinum toxin there is a significant improvement in the execution of memory saccades in cervical dystonia patients, as well as significantly better results of depression, anxiety and cognition scales. CONCLUSION: Patients with cervical dystonia have impaired smooth eye movements, saccades, antisaccades and voluntary saccades as well as pronounced non-motor symptoms (depression, anxiety, autonomic dysfunctions and cognition) compared to the healthy control group. After administration of botulinum toxin there is an independent improvement of all nonmotor symptoms in comparison with motor symptoms and memory-guided saccades. These results confirm the hypothesis of central action of botulinum toxin.