Objectives: The objective of this study was to determine the quality of laboratory services in a clinical laboratory at the primary health care level in the city of Osijek, from the point of view of their patients. Participants and methods: 400 patients (252 women and 148 men) were included in this research study. The study was organized on the principle of a cross-sectional study. The survey was conducted in the period from October 1, 2016 to February 28, 2017, with a response rate of 80 % (400 out of 500). A specially designed questionnaire and a standardized Servqual questionnaire were used in the study to assess the quality of laboratory services. Original written applications for databases and the statistical package Statistica for Windows 2010 were used for the statistical analysis of data. Results: In addition to patients’ socio-demographic and medical histories, the research study has established the rating of the functional quality, technical quality, and overall quality of the laboratory in Osijek. Patients in Osijek rated the quality of laboratory services with a high grade of 6.1 on a Likert scale of 1 to 7. Furthermore, the patients assessed the technical quality of the laboratory with a grade of 6.8, while the functional quality grade was lower, 5.9 (on a Likert scale of 1 to 7). Conclusion: The survey revealed the high quality assessment of laboratory services in clinical laboratory at the primary level of health care in the city of Osijek by its users.