Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro

Macan, Marija

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undergraduate thesis

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Effects of new ferrocene derivatives on the tumor cells growth

Marija Macan (2017)

Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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APA 6th Edition

Macan, M. (2017). Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:766132

MLA 8th Edition

Macan, Marija. "Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:766132

Chicago 17th Edition

Harvard

Macan, M. (2017). 'Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 23 July 2019, https://urn.nsk.hr/urn:nbn:hr:152:766132

Vancouver

Macan M. Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2019 July 23] Available at: https://urn.nsk.hr/urn:nbn:hr:152:766132

IEEE

M. Macan, "Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:766132

Cite this item: https://urn.nsk.hr/urn:nbn:hr:152:766132

Metadata

Title	Utjecaj novosintetiziranih derivata ferocena na rast tumorskih stanica in vitro
Title (english)	Effects of new ferrocene derivatives on the tumor cells growth
Author	Marija Macan
Mentor	Ljubica Glavaš-Obrovac (mentor)
Committee member	Saška Marczi (predsjednik povjerenstva)
Committee member	Barbara Viljetić (član povjerenstva)
Committee member	Ljubica Glavaš-Obrovac (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Faculty of Medicine Osijek Osijek
Defense date and country	2017-09-29, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Medical Biochemistry
Abstract	Uvod: Organometalni spojevi se zbog svojih svojstava mogu koristiti u liječenju različitih bolesti poput tumora i malarije. Cilj: ispitati antiproliferativni potencijal derivata ferocena na rast normalnih i tumorskih stanica u uvjetima in vitro. Definirati derivat koji ima najveću učinkovitost na rast tumorskih stanica a da istodobno ne djeluje inhibitorno na rast kulture normalnih stanica. Materijali i metode: Primijenjene su stanične linije u suspenziji (Raji i K562) i adherentne stanične linije (HeLa, CaCo-2 i MDCK). Stanične linije inkubirane su na temperaturi 37°C uz 5% CO2. Nakon obrade stanica derivatima ferocena (x-32, x-35, HŠ-11-1 i AM381) i inkubacije od 72 sata, uvid u broj živih stanica određivao se MTT testom. Rezultati: Najveći inhibitorni učinak na staničnim linijama pokazali su derivati x-32 i x-35, a uočeno je da je najosjetljivija stanična linija K562. Zaključak: Derivati ferocena uzrokuju smrt tumorskih stanica i smanjuju njihov rast. Postotak inhibicije ovisi o samoj koncentraciji spoja i o staničnoj liniji.
Abstract (english)	Introduction: Organometallic compounds due to their properties, can be used in the treatment of various diseases, such as cancer and malaria. Objective: The aim of the study is to examine the antiproliferative potential of ferrocene derivatives on the growth of normal and tumor cells under in vitro conditions. To define the derivatives that has the highest tumor cell growth performance while simultaneously not inhibiting the growth of normal cell cultures. Materials and Methods: The cell lines in suspension (Raji and K562) and adherent cell lines (HeLa, CaCo-2 and MDCK) were used in this research. Cell lines were incubated on the temperature of 37°C with 5% CO2. After treating the cells with the ferrocene derivatives (x-32, x-33, x-34, x-35, HŠ-11-1 and AM381) and incubation for 72 hours, the number of viable cells was determined by MTT test and measuring their apsorbance on spectrophotometer. Results: The highest inhibitory effect on cell lines were derivatives x-32 and x-35, while the most sensitive cell line was K562. Conclusion: Ferrocene derivatives cause the death of tumor cells and reduce their growth. The percentage of inhibition depends on the concentration of the compound and the type of the cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:152:766132
Study programme	Title: Medicinsko laboratorijska dijagnostika Study programme type: sveučilišni Study level: preddiplomski Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2018-04-09 11:21:36