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undergraduate thesis
Imunohistokemijske metode u dijagnostici tumora bubrega
2017. urn:nbn:hr:152:302733
Galinec, Magdalena
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Galinec, M. (2017). Imunohistokemijske metode u dijagnostici tumora bubrega (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:302733

Galinec, Magdalena. "Imunohistokemijske metode u dijagnostici tumora bubrega." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:302733

Galinec, Magdalena. "Imunohistokemijske metode u dijagnostici tumora bubrega." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:302733

Galinec, M. (2017). 'Imunohistokemijske metode u dijagnostici tumora bubrega', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 October 2024, https://urn.nsk.hr/urn:nbn:hr:152:302733

Galinec M. Imunohistokemijske metode u dijagnostici tumora bubrega [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2024 October 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:302733

M. Galinec, "Imunohistokemijske metode u dijagnostici tumora bubrega", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:302733

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Metadata
TitleImunohistokemijske metode u dijagnostici tumora bubrega
Title (english)Immunohistochemical methods in the diagnosis of renal tumors.
AuthorMagdalena Galinec
MentorMilanka Mrčela (mentor)
Committee memberTatjana Belovari (predsjednik povjerenstva)
Committee memberKsenija Marjanović (član povjerenstva)
Committee memberMilanka Mrčela (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2017-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pathology
Abstract
Cilj istraživanja: Ciljevi rada su bili prikazati rutinska imunohistokemijska bojenja koja se koriste u dijagnostici tumora bubrega, njihovu ekspresiju usporediti s pozitivnom probom te utvrditi broj i vrstu dijagnosticiranih tumora bubrega u 2015. i 2016. godini. Materijali i metode: Materijale čine arhivski uzorci tumora bubega. Svi su pregledani u medicinskoj dokumentaciji, a oni na kojima je provedeno imunohistokemijsko bojenje su analizirani. Mikroskopirani uzorci su prethodno obrađeni standardnim histokemijskim postupkom, bojani HE i obrađeni indirektom imunohistokemijskom procedurom s protutijelima za RCCm, CD10, CD117, CK7, Vimentin. Ekspresija imunohistokemijskog bojenja je opisana u odnosu na pozitivnu probu. Rezulatati : U ovom dvogodišnjem razdoblju dijagnosticirano je 86 slučajeva tumora bubrega od kojih njih 64 (74,42 %) pripada tipu svjetlostaničnog KBS-a. U dokumentaciji je opisano 18 svjetlostaničnih KBS-ova II. gradusa, a kod 14 slučajeva svjetlostaničnih KBS-ova gradus nije bio opisan. Imunohistokemijskim bojenjem je obrađeno samo 12 (13,95 %) slučajeva tumora bubrega. Vimentin je na najviše preparata bio jače eksprimiran od pozitivne probe (63,64 %), dok je CD117 u čak 7 (70,00 %) slučajeva bio negativan. Jednaka eksprimiranost tumorskog markera i pozitivne probe je zabilježena u samo 6 (10,71 %) slučajeva. Zaključak: Može se zaključiti da je svjetlostanični KBS najdijagnosticiraniji KBS u 2015. i 2016. godini. Ostali tipovi tumora bubrega kao što je papilarni KBS, kromofobni KBS i bubrežni onkocitom su značajno manje zastupljeni. Također, evidentno je da se imunohistokemijsko bojenje ne provodi često u dijagnostici tumora bubrega. U slučajevima kada je imunohistokeija provedena, ekspresija tumorskih markera je bila varijabilna. Ključne riječi: karcinom bubrežnih stanica; imunohistokemija; tumorski markeri; RCCm
Abstract (english)
Research goal: The goals were to present routine immunohistochemical staining which is used in kidney tumour diagnostics, but also to compare its expression with positive control and to determine the number and type of kidney tumours diagnosed in 2015 and 2016. Materials and methods: The materials consisted of archived samples of tumours. They were all examined in the medical documentation, and those which were used in immunohistochemical staining were completely analysed. Microscopical samples had been previously processed by standard histochemical procedure, stained with HE and antibodies for RCCm, CD10, CD117, CK7, Vimentin. Expression of immunohistochemical staining was described in comparison to positive control. Results: In the period of two years, 86 cases of kidney tumour were diagnosed. 64 of them (74.42%) belonged to specific type of tumours called clear cell RCC. In the documentation, only 18 cases of grade II clear cell RCC were described, while 14 cases were not described. Only 12 cases were processed by immunohistochemical staining. Vimentin was in the highest number of samples more expressed than positive control,while CD117 was shown as negative in 7 cases (70.00%). Equal expression of tumour markers and positive control was recorded only in 6 cases (10.71%). Conclusion: It can be concluded that clear cell RCC was the most diagnosed tumour in 2015 and 2016. Other types of kidney tumours, such as papillary RCC, chromophobe RCC and renal oncocytoma were less represented than clear cell RCC. In addition, it is evident that immunohistochemical staining is not present enough in kidney tumour diagnostics. In cases where immunohistochemistry was carried out, expressionof tumour markers was variable.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:302733
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-04-24 11:15:58