OBJECTIVES: The objectives of the study were to examine the effectiveness of transscleral cyclophotocoagulation with patients that have secondary neovascular glaucoma and to determine the level of pain during and immediately after the procedure. STUDY DESIGN: prospective study PARTICIPANTS AND METHODS: There were 36 participants in this study, all of whom suffered from refractory secondary neovascular glaucoma and who were subjected to transscleral cyclophotocoagulation from October 2017 until June 2018 at the Ophthalmology Institute CHC Osijek. Excluding criteria was previous procedure on the eye which had the aim of lowering intraocular pressure. All of the participants had their pressure measured with the Goldmann Applanation Tonometer before the procedure as well as on the first, the seventh, and the thirtieth day after the procedure. After the procedure, the participants rated the feeling of pain on the VAS scale. RESULTS: Prospective research included 36 participants (14 women and 22 men). Median age was 74. Statistically significant decrease of the intraocular pressure compared to the pressure before the procedure (Friedman's test, P < 0.001) was noted. There were no significant differences in the decrease of the intraocular pressure in relation to the operated eye and gender. Median of pain is 3, without significant difference in the severity of the pain in relation to the operated eye and gender. CONCLUSION: Transscleral cyclophotocoagulation is a non-invasive and safe method of treatment that quickly and effectively decreases the intraocular pressure with refractory secondary neovascular glaucoma.