Research goals. To explore and compare the knowledge and opinions of nursing students from different years of study about amniocentesis and whether they would recommend the procedure as practitioners. To examine the degree of awareness of nursing students of amniocentesis and their opinions in relation to this procedure. Study plan. Cross-sectional research was conducted at the Faculty of Medicine in Osijek in September 2017. Respondents and methods. The research was attended by students of the first and third years of undergraduate study of nursing. Data was collected by means of an anonymous questionnaire. Results. Data was processed for 47 respondents. It was found that some students of the first year of nursing are not fully acquainted with the procedure and purpose of amniocentesis, nor with fetal loss rates in case of complications. Most respondents believe that pregnant women should not be subjected to amniocentesis if there is no family history of chromosomal abnormalities. There is a consensus about the fact that amniocentesis reveals the possibility of Down syndrome at the early stage of pregnancy, accompanied by the view that amniocentesis procedure is not favoured for ethical reasons. When asked whether they would undergo the procedure themselves or recommend it, the majority were positive about amniocentesis, but were not entirely sure of giving their recommendation to other people. Also, the research shows lack of knowledge about the very medical procedure in first year students, and an increasingly significant difference of opinions in students in their third year of study . Conclusion. The lack of student knowledge in their first year of nursing studies on the procedure and purpose of amniocentesis leads to significant differences in survey results compared to third year students whose knowledge on the subject matter had been partly acquired during the course of the study.