| Abstract | Uvod: Polifenoli su biološki aktivni nenutritivni sastojci, sekundarni metaboliti biljnog metabolizma – fitokemikalije. Nastaju kao derivati fenilalanina s aromatskim prstenom i reaktivnom hidroksilnom grupom. Interes za polifenolima je vrlo velik zbog njihove raznolike biološke aktivnosti; djeluju antioksidativo, protutumorski, protuupalno, anti-alergijski, djeluju kao antibiotici, antilipidemici, opuštaju krvne žile i sprječavaju stvaranje krvnih ugrušaka. Cilj: Cilj ovog istraživanja je ispitati antiproliferativnu aktivnost polifenolnih spojeva izoliranih iz grožđanog tropa prije i nakon djelovanja mikroorganizama, koji potiču razgradnju grožđanog tropa, na kolorektalnim tumorskim stanicama in vitro. Materijali i metode: U istraživanju je upotrijebljen ekstrakt čistih polifenola (PP) i ekstrakti polifenola dobiveni nakon djelovanja mikroorganizma: Phanerochate chrysosporium (PC) i Trametes gibosa (TG). Korištene su dvije tumorske stanične kulture; CaCo-2 i SW 620. Stanice su uzgojene u CO2 inkubatoru (5% CO2/37°C) u DMEM HG mediju. Proliferativni učinak ekstrakta polifenola ispitan je primjenom kolorimetrijskog MTT testa.. Rezultati su obrađeni u Excelu i Statistika 13.1 programu primjenom Mann-Whitney U-testom (p<0,05). Rezultati: CaCo-2 stanice su pokazale najveću osjetljivost na djelovanje PP ekstrakta pri nižoj koncnentraciji (1,0 mg/ml) uz inhibiciju staničnog rasta od 56,6% (p<0,05), dok je pri koncentraciji od 2,5 mg/ml preživljenje 26,8% (PC), 36,8% (PP), 41,2% (TG). Učinak polifenolnih ekstrakata na SW620 stanice je najizražajniji pri koncentraciji 1,75 mg/ml i inhibiciju od 62,9-77,9% u nizu kako slijedi: PP > TG > PC (p<0,05) Zaključak: SW620 stanična linija je osjetljivija od CaCo-2 stanične linije na djelovanje polifenolnih ekstrakata. Razgradnja grožđanog tropa posredstvom mikroorganizama (TG, PC) nije značajno doprinijela antiproliferativnoj polifenolnoj aktivnosti ispitivanih uzoraka. |
| Abstract (english) | Introduction: Polyphenols are biologically active non-nutritive ingredients, secondary metabolites of plant metabolism - phytochemicals. They are formed as phenylalanine derivatives with an aromatic ring and a reactive hydroxyl group. The interest in polyphenols is very high due to their diverse biological activity; they have antioxidative, antitumor, anti-inflammatory, anti-allergic properties, they act as antibiotics and antilipidemics, they relax the blood vessels and prevent blood clots. Objective: The aim of this study is to investigate antiproliferative activity of polyphenolic compounds isolated from grape trope before and after the action of microorganisms, which promote the degradation of grape trout, on colorectal tumor cells in vitro. Materials and Methods: An extract of pure polyphenolic components and extracts of polyphenols obtained after the action of the Phanerochate chrysosporium (PC) and Trametes gibosa (TG) microorganisms were used in the study. Two tumor cell lines were used; CaCo-2 and SW 620. The cells were cultured in a CO2 incubator (5% CO2 / 37 ° C) in DMEM HG medium. The proliferative effect of the polyphenolic extracts was tested using a colorimetric MTT assay. The results were processed in Excel and Statistics 13.1 using the Mann-Whitney U-test (p <0.05). Results: Caco-2 cells showed the highest susceptibility to the effect of PP extract at lower concentrations (1.0 mg / ml) with 56.6% (p <0.05) cellular inhibition, while at a concentration of 2.5 mg / ml survival was 26.8% (PC), 36.8% (PP), 41.2% (TG). The effect of polyphenolic extracts on SW620 cells is most pronounced at 1.75 mg / ml and inhibition of 62.9-77.9% in the sequence as follows: PP> TG> PC (p <0.05). Conclusion: The SW620 cell line is more sensitive than Caco-2 to polyphenolic extracts. The degradation of grape trope by microorganisms (TG, PC) did not significantly contribute to the antiproliferative activity of the investigated samples. |
