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undergraduate thesis
Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora
2018. urn:nbn:hr:152:643170
Ančić, Elena
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Ančić, E. (2018). Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:643170

Ančić, Elena. "Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:643170

Ančić, Elena. "Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:643170

Ančić, E. (2018). 'Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:643170

Ančić E. Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:643170

E. Ančić, "Uloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:643170

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Metadata
TitleUloga Tempol-a na protokom potaknutu dilataciju nakon akutnog izlaganja hiperbaričnom kisiku zdravih Sprague-Dawley štakora
Title (english)Role of Tempol on the flow-induced dilation after acute hyperbaric oxygenation in healthy Sprague-Dawley rats
AuthorElena Ančić
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
CILJ: Glavni cilj ovog istraživanja je odrediti učinak Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora izloženih akutnoj hiperbaričnoj oksigenaciji. MATERIJALI I METODE: U istraživanje su bili uključeni zdravi muški Sprague Dawley štakori, podijeljeni u dvije skupine: CTRL skupina - zdravi netretirani štakori (N=6) te A_HBOT skupina - štakori izloženi akutnom djelovanju hiperbaričnog kisika u barokomori (100 %-tni kisik; 2 bar/2 sata, N=5). Prije dekapitacije, štakori su anestezirani s ketaminom (75 mg/kg) i midazolamom (0,5 mg/kg). Srednje cerebralna arterija je izolirana i inkubirana pri tlaku od ∆80 mmHg. Zatim se mjerio promjer ovisno o tlačnim gradijentima (∆10, ∆20, ∆40, ∆60 i ∆100 mmHg), prvo bazalne vrijednosti, a onda u prisutnosti Tempola (100 µmol/L). Na kraju eksperimentalnog protokola, PSS otopinu u sustavu zamijenili smo sa PSS Ca-free otopinom, kako bi se izmjerio maksimalni promjer krvne žile. REZULTATI: Rezultati pokazuju da in vitro primjena Tempola poboljšava razinu dilatacije u eksperimentalnoj skupini životinja koje su bile izložene akutnoj hiperbaričnoj oksigenaciji, dok u kontrolnoj skupini Tempol nije imao učinka na promjenu razine dilatacije središnje moždane arterije. ZAKLJUČAK: Tempol oporavlja narušenu protokom potaknutu dilataciju kod životinja koje su bilo izložene akutnom djelovanju hiperbaričnog kisika, smanjujući razinu stvorenog oksidativnog stresa, dok in vitro primjena Tempola kod zdravih netretiranih životinja ne dovodi do promjene razine dilatacije središnje moždane arterije.
Abstract (english)
Aim: The main goal of this study is to determine the effect of Tempol on the flow-induced dilation of Sprague-Dawley rats exposed to acute hyperbaric oxygenation. Materials and methods: The study included healthy male Sprague Dawley rats, divided into two groups: CTRL group – heatlhy untreated rats (N=6) and A_HBOT group - rats exposed to acute hyperbaric oxygen in hyperbaric chamber (100 % oxygen; 2 bar/2 hours, N=5). Before decapitation, rats were anesthetized with ketamine (75 mg/kg) and midazolam (0.5 mg / kg) anesthesia. The middle cerebral artery was isolated and incubated at a pressure of 80 mmHg. The diameter was measured depending on the pressure gradients (∆10, ∆20, ∆40, ∆60 i ∆100 mmHg), first basal values, and then with Tempol (100 μmol / L). At the end of the experimental protocol, PSS solution in the system was replaced with PSS Ca-free solution so the maximum blood vessel diameter could be measured. Results: The results show that Tempol in vitro improves the level of dilation in the experimental group of animals that were exposed to acute hyperbaric oxygenation, while in the control group of healthy untreated rats Tempol had no effect on flow-induced dilation of middle cerebral artery. Conclusion: Tempol restores flow-induced dilatation of middle cerebral artery in animals that were exposed to activity of acute hyperbaric oxygen by reducing the level of produced oxidative stress. Tempol in vitro has no effect on flow-induced dilation of middle cerebral arter in healthy untreated control rats.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:643170
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-01-11 09:10:31