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undergraduate thesis
Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa
2018. urn:nbn:hr:152:340002
Kovač, Ivana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kovač, I. (2018). Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:340002

Kovač, Ivana. "Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:340002

Kovač, Ivana. "Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:340002

Kovač, I. (2018). 'Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 October 2024, https://urn.nsk.hr/urn:nbn:hr:152:340002

Kovač I. Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 October 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:340002

I. Kovač, "Ekspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:340002

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Metadata
TitleEkspresija markera upale u masnom tkivu mužjaka Sprague Dawley štakora nakon kroničnog stresa
Title (english)The expression of inflammation markers in fatty tissue of male Sprague Dawley rats following chronic stress
AuthorIvana Kovač
MentorMarija Heffer (mentor)
Committee memberTatjana Belovari (predsjednik povjerenstva)
Committee memberRobert Selthofer (član povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-01-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Ciljevi istraživanja: pokazati razlike u veličini adipocita i pojavi upale u masnom tkivu mladih i starih ženki štakora nakon izlaganja lažnom i pravom kroničnom stresu. Nacrt studije: studija je provedena kao istraživanje parova. Materijali i metode: u studiju je bilo uključeno 40 ženki Sprague Dawley štakora (10 po skupini) koje su u dobi od 3,5 mjeseci ili 14,5 mjeseci izložene lažnom ili pravom kroničnom stresu u trajanju od 3 ciklusa po 10 dana. Fiksirano tkivo se krioprotektiralo ili uklopilo u parafin. Parafinski blokovi masnog tkiva izrezali su se na debljinu od 6 μm, stavili na stakla, deparafinizirali te obojali hemalaun/eozinom za određivanje površine pojedinih adipocita. Stupanj upale određivali smo imunohistokemijom i bojenjem retikulina po Gomoriju. Dehidrirani i pokriveni preparati pod mikroskopom su se fotografirali, a kvantifikacija se napravila u programu ImageJ. Rezultati: starije stresirane ženke štakora imale su statistički značajno veći volumen adipocita i stupanj upale nego ostale skupine. Broj M1 makrofaga smanjio se u mlađih životinja nakon kroničnoga stresa, dok je u starijih znatno narastao. Broj M2 makrofaga se smanjio nakon kroničnoga stresa i u mladih i u starih životinja. Zaključak: kronični stres imao je suprotan učinak na mlade i stare životinje. Dok je u mlađih životinja smanjio upalu niskog stupnja u masnom tkivu, u starih se životinja upala povećala.
Abstract (english)
Objectives: the aim of this study is to show differences in the size of adipocytes and the appearance of inflammation in the adipose tissue of young and old female rats after the exposure to sham and real chronic stress. Study design: the study was conducted as a matched pair study. Materials and Methods: the study included 40 female Sprague Dawley rats (10 per group) who were 3,5 months or 14,5 months exposed to sham or real stress for 3 cycles per 10 days. Fixed tissue was cryoprotected or embedded in paraffin. Paraffin blocks of adipose tissue were cut to 6 μm thick slices, placed on the slides, deparaffinized and stained hemalaun/eosin to determine the surface area of adipocytes. The degree of inflammation was determined by immunohistochemistry and staining on reticulin according to Gomori. Dehydrated and covered preparations were photographed under a microscope, and quantification was made using ImageJ. Results: older stressed female rats had statistically significantly larger adipocytes and higher degree of inflammation than other groups. Number of M1 macrophages decreased in younger animals after chronic stress, while in the elderly it increased significantly. Number of M2 macrophages decreased after chronic stress in both young and old animals. Conclusion: chronic stress had the opposite effect on young and old animals. While in younger animals it reduced low-grade inflammation in adipose tissue, in older animals inflammation increased.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:340002
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-01-11 12:29:35