Goal: The goal of this research is to determine socio-demographic characteristics of healthcare students attending the University of Osijek and their opinions on folk medicine. Study design: Cross-sectional study Subjects and methods: The research was conducted on healthcare students of the Josip Juraj Strossmayer University of Osijek, meaning students of the Faculty of Medicine and of the Faculty of Dental Medicine and Health in Osijek. In the research, 200 second-year students of undergraduate and integrated study programs and 206 first-year students of graduate and fourth-year students of integrated study programs participated. They filled out a 15 minute anonymous online questionnaire during the period from May 2 to June 2, 2019. Results: Female students are significantly more familiar with the term, as well as the practical applications of folk medicine, than male students. Students with higher monthly income in their households use folk medicine preparations more frequently. First-year students of graduate and fourth-year students of integrated study programs consider significantly more that terms folk medicine, traditional, natural and alternative medicine are not synonyms. Students of lower years of study programs believe considerably more that folk medicine is usually used by people with lower levels of education. Conclusion: Healthcare students from the University of Osijek are relatively well familiar with the term folk medicine, with slight divergence of opinions, given their socio-demographic characteristics. If possible, attending a formal education on this subject would be a great option for students so they could have even wider knowledge for their future professional career.