prikaz prve stranice dokumenta ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA
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ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA
2019. urn:nbn:hr:152:844803
Takač, Boris
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Cite this document

Takač, B. (2019). ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA (Doctoral thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:844803

Takač, Boris. "ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:844803

Takač, Boris. "ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:844803

Takač, B. (2019). 'ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA', Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 October 2024, https://urn.nsk.hr/urn:nbn:hr:152:844803

Takač B. ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA [Doctoral thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 October 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:844803

B. Takač, "ULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA", Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:844803

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TitleULOGA INTERLEUKINA 6, GENSKIH POLIMORFIZAMA INTERLEUKINA 6, TRANSKRIPCIJSKOG ČIMBENIKA STAT3 I RECEPTORA ZA INTERLEUKIN 23 U CROHNOVOJ BOLESTI I ULCEROZNOM KOLITISU UZ DJELOVANJE C-REAKTIVNOG PROTEINA
Title (english)The Role of Interleukin 6, Polymorphisms of Interleukin 6, Transcription Factor STAT3 and Interleukin 23 Receptor and their Relationship with C-Reactive Protein in Crohn's Disease and Ulcerative Colitis
AuthorBoris Takač
MentorSilvio Mihaljević (mentor)
Committee memberJerko Barbić (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Cilj istraživanja: Crohnova bolest i ulcerozni kolitis obilježeni su kroničnom upalom crijeva nepredvidiva tijeka koja je imunološki posredovana. U uvjetima dugotrajne upale moguć je razvoj prekancerogenih displastičnih lezija i karcinoma crijeva povezanih s mjestima aktivne upale. Cilj ovoga istraživanja bio je ispitati učinke citokina, biokemijskih biljega i polimorfizama gena na razvoj CB-a, UC-a i UBC-a općenito te utvrditi razlike između UBC i kontrolne skupine ispitanika. Nacrt studije: U 143 ispitanika, podijeljenih s obzirom na dijagnostičke kriterije u CB i UC skupinu ispitanika, te 100 ispitanika kontrolne skupine izmjerene su serumske koncentracije IL-6 i CRP-a te su određene genske inačice gena čiji se genski produkti smatraju posrednicima upale (IL-6) ili su povezani s proupalnim signaliziranjem (STAT3 i IL-23R). Studija je ustrojena kao istraživanje parova. Materijal i metode: Serumska koncentracija IL-6 određena je kemiluminiscentnom sekvencijalnom imunometričnom, a koncentracija CRP-a imunoturbidimetrijskom metodom. Analiza polimorfizama gena IL-6 rs1800795, IL-23R rs11209026 i rs7530511 i STAT3 rs744166 provodila se na uređaju LightCyclerTM PCR 1.2 u stvarnome vremenu temeljem prijenosa energije uslijed fluorescentne rezonancije. Rezultati: Serumske koncentracije IL-6 bile su statistički značajno više u UBC skupini u usporedbi s kontrolnom skupinom, uz pozitivnu korelaciju s kliničkom aktivnosti bolesti. Koncentracija IL-6 nije se razlikovala između dobnih skupina UBC ispitanika kao ni između žena i muškaraca. Serumske koncentracije CRP-a više su u CB i UC populaciji ispitanika od referentnog intervala. Utvrđena je pozitivna korelaciju CRP-a s indeksom aktivnosti UC-a mjerenoj prema skali MTWSI i serumskom koncentracijom IL-6. Koncentracija CRP-a viša je kod CB ispitanika bez intestinalne resekcije obzirom na CB ispitanike koji su prethodno operativno liječeni. Kod IL-6 nisu uočene razlike između ovih dvaju skupina ispitanika. Serumske koncentracije IL-6 i CRP-a kod CC genotipa IL-6 značajno su više kod UC ispitanika s obzirom na GG + GC genotip. Polimorfizam IL-23R rs11209026 povezan je s UBC-om za razliku od rs7530511 kod kojega nije utvrđena statistički značajna razlika. STAT3 rs744166 ne pokazuje statistički značajnu razliku distribucije genotipa između UBC i kontrolne skupine ispitanika. Nisu utvrđene statistički značajne interakcije između odabranih genskih inačica u UBC-u. Zaključak: Serumske koncentracije IL-6, CRP-a te polimorfizam IL-23R rs11209026 imaju utjecaj na sklonost pobolu i obilježju UBC-a. CC genotip IL-6 povezan je s višom koncentracijom IL-6 i CRP-a kod UC-a obzirom na GG + GC genotip. IL-23R rs11209026 ima zaštitni učinak za CB dok polimorfizmi IL-23R rs7530511 i STAT3 rs744166 ne pokazuju povezanost s CB-om i UC-om kao ni s UBC-om općenito.
Abstract (english)
Objectives: Crohn's disease (CD) and ulcerative colitis (UC) are marked by chronic immunologically mediated intestinal inflammation with an unpredictable disease course. Prolonged inflammation can lead to precancerous lesions and colon cancer associated with active inflammation sites. The present study aims at exploring the effects of cytokines, biochemical markers and gene polymorphisms on CD, UC and inflammatory bowel diseases (IBDs) overall and determine the differences between IBD patients and healthy controls. Study Design: IL-6 and CRP levels were measured and genetic variants whose genetic products are considered inflammatory mediators (IL-6) or are associated with proinflammatory signalling (STAT3 and IL-23R) in 143 patients diagnosed with CD or UC and 100 healthy controls. A paired research design was applied in the study. Material and Methods: Quantitative assessment of IL-6 and CRP sera was performed with solid-phase, enzyme-labeled, chemiluminescent sequential immunometric and immunoturbidimetric assay, respectively. A real-time fluorescence resonance energy transfer-based method in LightCyclerTM PCR 1.2 was used for genotyping of polymorphisms IL-6 rs1800795, IL-23R rs11209026 and rs7530511 and STAT3 rs744166 genes. Results: IL-6 serum levels were statistically significantly higher in IBD than in healthy controls and were positively correlated with clinical disease activity. IL-6 levels were not significantly different between age groups or male and female IBD patients. In CD and UC patients, CRP serum levels were higher than the reference intervals. Positive correlations between CRP and IL-6 serum levels and CRP serum levels and UC activity index as measured by MTWSI were observed. CRP serum level was higher in CD patients without intestinal resection than in CD patients with prior intestinal resection. No differences were observed in IL-6 serum levels in the two patient groups. In UC patients, IL-6 and CRP serum levels are statistically significantly higher in CC IL-6 genotype in comparison to GG+GC genotype. Polymorphism IL-23R rs11209026 is associated with IBD, unlike rs7530511 for which no statistically significant difference was observed. For STAT3 rs744166 no statistically significant difference in genotype distribution was observed between IBD and healthy controls. No statistically significant interactions between selected gene variants were found in IBD. Conclusion: IL-6, CRP serum levels and IL-23R rs11209026 polymorphism affect the tendency to morbidity and characteristics of IBD. The CC IL-6 genotype is associated with higher IL-6 and CRP serum levels than GC+CC genotype in UC patients. IL-23R rs11209026 has a protective effect in CD, while no association was observed between IL-23R rs7530511 and STAT3 rs744166 polymorphisms and CD, UC or IBD overall.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:844803
Study programmeTitle: University Postgraduate Study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-12-19 12:44:40