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Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro
2020. urn:nbn:hr:152:346511
Jeleč, Antonela
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Jeleč, A. (2020). Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:346511

Jeleč, Antonela. "Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:346511

Jeleč, Antonela. "Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:346511

Jeleč, A. (2020). 'Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 01 April 2025, https://urn.nsk.hr/urn:nbn:hr:152:346511

Jeleč A. Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2025 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:152:346511

A. Jeleč, "Procjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:346511

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Metadata
TitleProcjena antiproliferativne aktivnosti paladijevih (II) kompleksa in vitro
Title (english)Evaluation of the antiproliferative activity of palladium (II) derivatives in vitro
AuthorAntonela Jeleč
MentorKatarina Mišković Špoljarić (mentor)
Committee memberTeuta Opačak-Bernardi (predsjednik povjerenstva)
Committee memberSaška Marczi (član povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Maligna oboljenja su drugi vodeći uzrok smrti na globalnoj razini. S obzirom na veliki broj smrti uzrokovanih malignim promjenama, potražnja za antitumorskim lijekovima je vrlo velika. Jedan od često primijenjenih kemoterapeutika je cis-platina, međutim primjena cis-platine pokazala je mnoge nuspojave. Stoga se pažnja usmjerava na paladij koji stvara komplekse kao i platina te se stoga može zamijeniti istom. Ciljevi: Ispitati citotoksični učinak paladijevih (II) kompleksa na rast četiriju... More staničnih linija (PANC1, BJ, MDCK.1, NCI-H358) u uvjetima in vitro. Materijali i metode: Paladijevi (II) kompleksi sintetizirani su na Prehrambeno tehnološkom fakultetu u Osijeku, Katedra za kemiju i ekologiju, pri Zavodu za primjenjenu kemiju i ekologiju. Spojevi su pripremljeni otapanjem u 100% DMSO u koncentraciji 10-2 mol/dm3 (M), a konačan niz koncentracija je bio 10-4 M, 10-5 M i 10-6 M. Kultivacija stanica odvijala se u CO2 inkubatoru pri 37°C. Stanična vijabilnost određena je MTT testom nakon inkubacije od 72 sata. Rezultati: Uočen je antiproliferativni učinak testiranih spojeva na stanične linije gdje je veća citotoksičnost primijećena pri većim koncentracijama ispitivanih kemijskih spojeva. Najučinkovitije antiproliferativno djelovanje pokazali su Pd 1 i Pd 4, na svim tumorskim stanicama, najbolje pri koncentraciji 10-4 M. Normalne stanice humanog podrijetla, BJ, pokazale su dobru otpornost na ispitivane spojeve uz postotak preživljenja od 50-65% pri najvišoj koncentraciji. Najveću osjetljivost pokazala je mišja, MDCK1 stanična linija. Zaključak: Paladijevi (II) kompleksi smanjuju rast tumorskih stanica. Inhibicija rasta ovisi o koncentraciji spoja i vrsti stanične linije. Less
Abstract (english)
Introduction: Malignant diseases are worlds second leading cause of death. The request for anti-tumor drugs is growing due to a large number of deaths caused by malignant changes. One of the most used chemotherapeutics is cisplatin. However, cisplatin use led to numerous side effects. Because of this, our attention is shifted to palladium, which forms complexes same as platinum, so may be a supstitute for platinum. Objective: To research the cytotoxic effect of palladium (II) complexes on the... More growth of four cell lines (PANC1, BJ, MDCK.1, NCI-H358) under in vitro conditions. Materials and methods: Palladium (II) complexes were synthesized at the Faculty of Food Technology in Osijek, Subdepartment of Chemistry and Ecology, at the Department of Applied Chemistry and Ecology. Compounds were made by dissolvment of 100% DMSO in concentration of 10-2 mol/dm3 (M), and final count of concentrations was 10-4 M, 10-5 M and 10-6 M. Cultivation of the cells was carried out in an CO2 incubator at 37°C. The cell viability was determined by MTT assay after 72-hour incubation. Results: An antiproliferative effect of the tested compounds on cell lines was observed. A higher cytotoxicity was noticed at higher concentrations of the tested chemical compounds. Most effective antiproliferative action were showed by Pd 1 and Pd 4, on all tumor cells at concentration of 10-4 M. Normal cells that originated from humans, BJ, showed a good resistance for tested samples and with survival percentage of about 50-65% within highest concentration. Largest sensitivity showed mouse MDCK1 colony. Conclusion: Palladium (II) complexes reduce the growth of tumor cells. The inhibition of growth depends on the concentration of the compound and the cell line type. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:346511
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2020-10-26 13:17:25
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