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undergraduate thesis
Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika
2020. urn:nbn:hr:152:592787
Šafar, Iva
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Šafar, I. (2020). Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:592787

Šafar, Iva. "Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:592787

Šafar, Iva. "Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:592787

Šafar, I. (2020). 'Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:592787

Šafar I. Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:152:592787

I. Šafar, "Utjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:592787

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TitleUtjecaj konzumacije kokošjih jaja obogaćenih n-3 polinezasićenim masnim kiselinama (n-3 PUFA) na izražaj gena antioksidativnih enzima u mononuklearnim stanicama krvi mladih zdravih ispitanika
Title (english)The effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) chicken eggs consumption on antioxidative enzymes' gene exrpression in mononuclear blood cells in young healthy individuals
AuthorIva Šafar
MentorInes Drenjančević (mentor)
Committee memberAna Stupin (predsjednik povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
UVOD: Oksidativni stres u podlozi je mehanizama poremećene vaskularne reaktivnosti koja je povezana s razvojem kardiovaskularnih bolesti. U prijašnjim istraživanjima (Appl Physiol Nutr Metab. 2018) prikazano je da postoji promjena razine oksidativnog stresa nakon konzumacije n-3 PUFA obogaćenih jaja kod mladih zdravih ispitanika. CILJ ISTRAŽIVANJA: Utvrditi izražaj gena za SOD1 - superoksid dismutazu, GPX1 - glutation peroksidazu, CAT - katalazu i MPO - mijeloperoksidazu u izoliranim mononuklearnim stanicama periferne (PBMC) krvi kod mladih zdravih ispitanika koji su konzumirali n-3 PUFA obogaćena jaja ili obična, kontrolna jaja prije i nakon dijetetskog protokola. NACRT STUDIJE: dvostruko slijepa, randomizirana MATERIJALI I METODE: Mononuklearne stanice periferne krvi izolirane su pomoću gradijenta gustoće. Iz njih je izolirana RNA pomoću TRIzol otopine te je određen genski izražaj antioksidativnih enzima pomoću PCR-a u realnom vremenu na uređaju Bio Rad CFX96. U istraživanju su sudjelovali mladi sedentarni ispitanici, oba spola. Za grupne usporedbe korišten je t-test, dok su se usporedbe unutar skupine provodile korištenjem uparenog t-testa ili Wilcoxon testa, statistička značajnost postavljena je kao p < 0,05. REZULTATI: Preliminarni rezultati genskog izražaja antioksidativnih enzima SOD1, CAT, GPx1 te MPO prije i poslije konzumacije n-3 PUFA obogaćenih jaja ne pokazuju značajne statističke razlike. ZAKLJUČAK: Nije moguće pravilno očitovati povoljan učinak omega-3 masnih kiselina na genski izražaj antioksidativnih enzima jer stanice prethodno nisu bile izložene stresoru.
Abstract (english)
INTRODUCTION: Oxidative stress underlies the mechanisms of impaired vascular reactivity associated with the development of the cardiovascular disease. Previous research (Appl Physiol Nutr Metab. 2018) has shown that there is a change in oxidative stress levels after consumption of n-3 PUFA enriched eggs in young healthy subjects. OBJECTIVES: The aim of this study was to determine the expression of genes for SOD1 - superoxide dismutase, GPX1 - glutathione peroxidase, CAT - catalase, and MPO - myeloperoxidase in isolated peripheral (PBMC) blood mononuclear cells in young healthy subjects who were controlled or consumed by PFA. eggs before and after the dietary protocol. STUDY DESIGN: Double-blind, randomized MATERIALS AND METHODS: Peripheral blood mononuclear cells were isolated using a density gradient. RNA was isolated from them using a TRIzol solution and the genetic expression of antioxidant enzymes was determined by real-time PCR on a Bio Rad CFX96 device. Young sedentary respondents, both sexes, participated in the study. For group comparisons, a t-test was used, while intra-group comparisons were performed using paired t-test or Wilcoxon test, statistical significance was set as p <0.05. RESULTS: Preliminary results of gene expression of antioxidant enzymes SOD1, CAT, GPx1, and MPO before and after consumption of n-3 PUFA enriched eggs do not show significant statistical differences. CONCLUSION: It is not possible to correctly demonstrate the beneficial effect of omega-3 fatty acids on the genetic expression of antioxidant enzymes because the cells have not been previously exposed to the stressor.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:592787
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2020-11-12 12:33:02