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undergraduate thesis
Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom
2019. urn:nbn:hr:152:823094
Poštić, Mirna
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Poštić, M. (2019). Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:823094

Poštić, Mirna. "Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:823094

Poštić, Mirna. "Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:823094

Poštić, M. (2019). 'Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 April 2024, https://urn.nsk.hr/urn:nbn:hr:152:823094

Poštić M. Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 April 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:823094

M. Poštić, "Stvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:823094

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Metadata
TitleStvaranje dušikovog oksida u krvnim žilama Spragu-Dawley štakora na terapiji Losartanom
Title (english)Production of nitric oxide in blood vessels of Sprague-Dawley rats on Losartan therapy
AuthorMirna Poštić
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj:Odrediti stvaranje NO-a u srednjoj moždanoj arteriji kod zdravih Sprague-Dawley (SD) štakora te odrediti utjecaj Losartana na stvaranje NO-a. Materijali i metode:U istraživanje su bili uključeni muški SD štakori koji su bili podijeljeni u dvije skupine: 1. skupina (CTRL) su bili zdravi netretirani štakori, a 2. skupina je (CTRL+Losartan) primala Losartan u vodi za piće kroz sedam dana. Prije dekapitacije štakori su anestezirani kombinacijom ketamina (75 mg/kg) i midazolama (0,5 mg/kg). Srednja cerebralna arterijaje izoliranate postavljena u sustav za izolirane žile u uvjetima bez (∆mmHg) / sa (∆80mmHg) protokom te uz prisutnost L-NAME-a. Proizvodnja NO-a je određena pomoću fluorescentne boje DAF-2DA i fluorescentnog mikroskopa.Svi eksperimentalni postupci su usklađeni s europskim smjernicama za skrb i primjenu laboratorijskih životinja (direktiva 86/609) te su poduzete sve mjere kako bi se spriječila patnja životinja. Navedena istraživanja su odobrena za provedbu od strane Etičkog povjerenstva Medicinskog fakulteta Osijek. Rezultati:Rezultati pokazuju da je stvaranje NO-a značajno smanjeno u CTRL+Losartan skupini u uvjetima bez protoka (no flow) uspoređujući s kontrolnom skupinom. U uvjetima prisutnog protoka (flow) nema značajne razlike u količini stvorenog NO-a između skupina. L-NAME značajno smanjuje razinu NO-a u obje ispitivane skupine i u uvjetima bez protoka te uz prisutan protok. Zaključak:Losartan značajno smanjuje proizvodnju NO-a u uvjetima bez protoka (no flow uvjeti) dok je uz prisutan protok (flow uvjeti) stvaranje NO-a podjednako kod kontrolnih štakora i kod onih na terapiji Losartanom. U oba uvjeta, bez i u prisutnosti protoka, L-NAME značajno snižava razinu stvorenog NO-a u obje skupine.
Abstract (english)
Aim:Present study aimed to determine the effects of Losartan therapy on flow/no flow-induced endothelial NO production, in healthy Sprague-Dawley (SD) rats. Materials and methods: Healthy male 11 weeks old Sprague-Dawley rats were divided in: CTRL (control group) and CTRL+Losartan group (received Losartan in drinking water for 7 days). Prior to decapitation, rats were anesthetized by ketamine (75 mg/kg) and midazolam (0.5 mg/kg). Middle cerebral arteries were isolated and cannulated and pressurized at ∆0mmHg or ∆80 (no flow/flow conditions), in the absence/presence of TEMPOL in vitro. NO production was determined by DAF-2DA to DAF-2T conversion fluorescence assay. All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by institutional Ethical Committee. Results:No flow NO production was significantly reduced in the CTRL+Losartan group comparing with the CTRL group, while flow-induced NO production was similar between CTRL and CTRL+Losartan group. L-NAME significantly reduces the level of NO production in both groups, and with/without flow conditions. Conclusion:Losartan significantly reduces the NO production in conditions without flow, while in flow conditions the NO production were similar between control rats and rats on Losartan therapy. In both conditions, with/without flow, L-NAME significantly reduces the level of produced NO in both groups.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:823094
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-11-20 13:02:33