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undergraduate thesis
Optimizacija izolacije mononuklearnih stanica periferne krvi
2019. urn:nbn:hr:152:734451
Šokčević, Anamaria
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Šokčević, A. (2019). Optimizacija izolacije mononuklearnih stanica periferne krvi (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:734451

Šokčević, Anamaria. "Optimizacija izolacije mononuklearnih stanica periferne krvi." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:734451

Šokčević, Anamaria. "Optimizacija izolacije mononuklearnih stanica periferne krvi." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:734451

Šokčević, A. (2019). 'Optimizacija izolacije mononuklearnih stanica periferne krvi', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 01 April 2025, https://urn.nsk.hr/urn:nbn:hr:152:734451

Šokčević A. Optimizacija izolacije mononuklearnih stanica periferne krvi [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2025 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:152:734451

A. Šokčević, "Optimizacija izolacije mononuklearnih stanica periferne krvi", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:734451

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Metadata
TitleOptimizacija izolacije mononuklearnih stanica periferne krvi
Title (english)Optimization of peripheral mononuclear blood cells isolation
AuthorAnamaria Šokčević
MentorŽeljko Debeljak (mentor)
Committee memberSaška Marczi (predsjednik povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
Committee memberŽeljko Debeljak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Cilj istraživanja: Optimizacija izolacije PBMC-a u svrhu postizanja što većeg iskorištenja i čistoće pripravaka Nacrt studije: Ova klinička opservacijska studija dio je šireg projekta optimizacije validacije kandidata za tumorske biljege karcinoma pluća ne-malih stanica. Materijali i metode: U istraživanju je sudjelovalo 40 ispitanika koji su bili podijeljeni u 4 skupine kako bi se ispitali različiti uvjeti pripreme izolata. Krv se uzorkovala u dvije epruvete; za izolaciju PBMC-a... More (komercijalna epruveta s gradijentom fikola) i za evaluaciju uspješnosti izolacije PBMC-a (epruveta s K3-EDTA). Parametri koji su ispitivani su duljina centrifugiranja i broj okretaja. Brojanjem stanica na hematološkom brojaču utvrđeni su optimalni uvjeti za izolaciju PBMC-a. Rezultati: Brzina centrifugiranja ne utječe značajno na broj izoliranih stanica i njihovo iskorištenje, dok vrijeme centrifugiranja utječe značajno na ukupan broj leukocita, limfocita i monocita. Također vrijeme centrifugiranja utječe značajno na % izoliranih stanica leukocita, limfocita i monocita. Duljina i brzina centrifugiranja ne utječu na broj trombocita i % izoliranih trombocita. Zaključak: Izolacija mononukleara optimizirana je na 30 minuta centrifugiranja pri 1600 RCF jer je pri tim uvjetima najveće iskorištenje PBMC-a i smanjena je kontaminacija izolata mononuklearnih stanica ostalim krvnim frakcijama. Less
Abstract (english)
Objectives: The aim of this research is to optimize the isolation of PBMCs in order to maximize the yield and purity of the preparations Study design: This clinical observational study is part of a wider project. The goal of that project is to optimize the validation of candidates for non-small cell lung cancer tumor markers. Materials and methods: The study involved 40 subjects (healthy volunteers) who were divided into 4 groups to examine different conditions of isolate... More preparation. Blood was sampled in two tubes; for PBMC isolation (a commercial Ficoll gradient tube) and for PBMC isolation performance evaluation (a K3-EDTA tube). The examined parameters were the centrifugation length and the relative centrifugal force. Optimal conditions for the isolation of PBMC are determined by counting cells in a hematology counter. Results: Centrifugation speed does not affect the number of cells isolated and their utilization, while centrifugation time significantly affects the total number of leukocytes, lymphocytes and monocytes. Also, centrifugation time affects on % of isolated cells of leukocytes, lymphocytes and monocytes. The length and speed of the centrifugation do not affect the platelet count and % of platelets isolated. Conclusion: Mononuclear cells isolation is optimized at 30 minutes of centrifugation at 1600 RCF because under these conditions the highest utilization of PBMCs was obtained and the contamination of the mononuclear cell isolate by other blood fractions was reduced. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:734451
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-11-26 09:47:02
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