Objectives: The aim of this study was to test the effect of newly synthesized 7-chloro-4-aminoquinoline derivatives on tumor cell growth and to determine the mechanisms by which treated cells die by investigating apoptosis and autophagy activity with flow cytometry. Study Design: The study was designed as a trial (experimental). Materials and Methods: The effect of 7-chloro-4-aminoquinoline derivatives was tested on tumor cells (MDA-MB-231, K-562, HuT 78) and on a normal cell line (MDCK 1). The inhibitory effect of quinoline derivatives on tumor cell growth was determined by the MTT assay. The proapoptotic potential of quinolines on tumor cells was determined by flow cytometry with prior staining of the cells with Annexin V FITC and propidium iodide, while their effect on autophagy was determined by flow cytometry on Acridine Orange-stained cells. For apoptosis and autophagy analysis, cells were treated with derivatives at a concentration of 5 x 10-6 M. Results: The tested quinoline derivatives showed cytotoxic effects on tumor cells, with a desirable relenting effect on normal cell line. By determining the proportion of cells in apoptosis by a flow cytometer, it was determined that the derivative MB-173 showed statistically significant (P < 0,05) proapoptotic effect on K-562 and MDA-MB-231 cell lines. The analysis of the effect of derivatives on autophagy revealed that the MB-173 derivative had a statistically significant (P < 0,05) effect on the autophagy of the MDA-MB-231 cell line. Conclusion: The tested derivatives showed a diminished effect on the normal cell line while inhibiting tumor cell growth, with HuT 78 and K-562 cell lines showing greater sensitivity to quinoline derivatives than MDA-MB-231 cells. Of the derivatives tested, MB-173 significantly induced apoptosis in the K-562 cell line and induced both apoptosis and autophagy in the MBA-MB-231 cell line. For better understanding of the antitumor mechanism of action of the tested quinoline derivatives, further research is needed.