Objectives: To compare the influence of commercially available mounting media on the display of the distribution of complex gangliosides, GPI-anchored proteins and transmembrane proteins and to determine the durability of the results after immunohistochemical analysis. Study Design: Experimental study on laboratory animals Material and methods: The brain of three month old mouses were used for this study. After sacrificing the animals, their brains were isolated, fixed and cryoprotected. Immunohistochemical analysis was performed using primary antibodies for gangliosid GD1a, Thy-1 protein and GABA receptor. The results were photographed by digital camera on a light microscope, analyzed in Fiji and statistics were produced in XLSTAT. Results: In the presentation of immunohistochemical staining in ganglioside GD1a, the greatest change occurred in the case of mounting media Fluromount, which was shown as statistically significant, especially in the ML and WM layers. At the GABA receptor, the greatest change in the display occured with mounting media DakoCytomation, which was shown by analysis to be statistically significant, especially in the ML layer and WM area. Immunohistochemical staining in Thy-1 protein changed most with mounting media DakoCytomation, which is statistically significant in the GL and WM layers. Conclusion: Different mounting media affect the results of immunohistochemical analysis so, to obtain accurate results, the photographs of specimens should be taken immediately under a microscope.