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master's thesis
Proapoptotički učinci derivata kumarina in vitro
2019. urn:nbn:hr:152:300410
Pnjak, Luka
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Pnjak, L. (2019). Proapoptotički učinci derivata kumarina in vitro (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:300410

Pnjak, Luka. "Proapoptotički učinci derivata kumarina in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:300410

Pnjak, Luka. "Proapoptotički učinci derivata kumarina in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:300410

Pnjak, L. (2019). 'Proapoptotički učinci derivata kumarina in vitro', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 18 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:300410

Pnjak L. Proapoptotički učinci derivata kumarina in vitro [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 December 18] Available at: https://urn.nsk.hr/urn:nbn:hr:152:300410

L. Pnjak, "Proapoptotički učinci derivata kumarina in vitro", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:300410

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Metadata
TitleProapoptotički učinci derivata kumarina in vitro
Title (english)Proapoptotic effects of coumarine derivatives in vitro
AuthorLuka Pnjak
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberTeuta Opačak-Bernardi (predsjednik povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Kumarin ili 2H-kromen-2-on je heterociklički aromatski kemijski spoj koji je široko rasprostranjen u prirodi. Maligne bolesti predstavljaju jedan od najvećih problema u današnjoj medicini, a kumarini nalaze ulogu u liječenju istih ili nuspojava uzrokovanih nekom drugom vrstom terapije malignih bolesti. Cilj: Ispitati utjecaj derivata kumarina na aktivaciju apoptoze i autofagije u tumorskim stanicama in vitro. Materijali i metode: Proapoptotički učinak derivata kumarina (DK1, DK2 i DK3), u koncentraciji od 1x10-5 M, ispitan je na HeLa i MCF-7 staničnoj liniji, a određen protočnim citometrom. Rezultati: Svi testirani derivati djeluju na aktivaciju apoptoze u HeLa stanicama dok na MCF-7 stanice značajno utječe samo derivat DK3. Utjecaj na autofagiju je snižen u odnosu na kontrolu u obje stanične linije. Zaključak: Testirani derivati pokazuju povećan apoptotički učinak na HeLa staničnu liniju. U obje stanične linije ne potiču autofagiju.
Abstract (english)
Introduction: Coumarin, or 2H-chromen-2-one, is a heterocyclic aromatic chemical compound that is widespread in nature. Malignancies are one of the biggest problems in today's medicine and coumarins find a role in treating the same or side effects caused by some other type of malignancy therapy. Objective: To examine the influence of coumarin derivatives on the activation of apoptosis and autophagy in tumor cells in vitro. Materials and methods: The proapoptotic effect of coumarin derivatives (DK 1, DK 2 and DK 3), in concentration of 1x10-5 M, was examined on a HeLa and MCF-7 cell line, determined by a flow cytometer. Results: All tested derivatives affect the activation of apoptosis in HeLa cells, while MCF-7 cells are significantly affected only by the DK 3 derivative. The effect on autophagy was reduced in regard to both cell lines. Conclusion: The derivatives tested show an increased apoptotic effect on the HeLa cell line. In both cell lines they do not promote autophagy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:300410
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-05-27 12:33:56