OBJECTIVES. The aim of this study was to determine is there any expression of TFF3 protein in primary skin tumours, whether there are significant differences in the expression of TFF3 protein in healthy skin and skin tumours and are there any significant differences between the different types of skin cancer. STUDY DESIGN. The samples were stained using immunohistochemistry, histomorphologically analyzed by light microscopy (qualitative) and the signal strength was semiquantitatively analyzed by counting TFF3 positive cells. MATERIAL AND METHODS. The study was conducted on a total of 53 samples, 12 samples of healthy skin, 14 samples of basal cell carcinoma, 15 samples of squamous cell carcinoma and 12 samples of malignant melanoma. Slides were processed and analyzed by immunohistochemistry using a light microscope. The obtained data were processed using statistical program MedCalc Statistical Software version 15.11.4 (MedCalc Software BVBA, Ostend, Belgium). RESULTS. Expression of TFF3 protein was noticed in primary skin tumours as well as in the epidermis of healthy skin by morphological analysis. The expression of TFF3 protein was significantly lower in basal cell and squamous cell skin cancer compared to healthy skin, unlike breast cancer where there is an increased expression of the TFF3 protein. Among the various types of skin cancer there was not a significant difference in the expression of proteins TFF3. CONCLUSION. TFF3 protein expression was present in primary tumours of the skin but no significant differentes were cound in staining intensity between different tumour types. TFF3 expression was significantly lower in the basal cell and squamous cell carcinoma than in healthy skin.