Objectives: The aim of this study was to examine the influence of gender on the age of onset of Parkinson's disease, non-motor symptoms and autonomic dysfunction. Study design: Cross-sectional study. Participants and methods: The study included 96 patients with idiopathic Parkinson's disease who are treated in the outpatient clinic for movement disorders of the Clinic of Neurology, University Hospital Center Osijek. The demographic questionnaire, the NMS questionnaire and the SCOPA-AUT questionnaire were used as research instruments. Results: The arithmetic mean of age of disease onset is 60.1 (standard deviation 10.72) years for women and 61.1 (standard deviation 10.59) years for men. There is no significant difference in age, age of onset and duration of the disease between the genders. Non-motor symptoms analyzed by the NMS questionnaire indicate a more frequent occurrence of urinary urgency (P = 0.05) and feelings of anxiety and panic (P = 0.008) in women and decreased or increased sexual desire in men (P = 0.05). There is no statistically significant gender difference for other non-motor symptoms. According to the results of the SCOPA-AUT questionnaire, there is a gender difference in thermoregulatory functions (P = 0.03). In the other five domains, as well as in the total score, no significant difference in autonomic symptoms between the genders was observed. Conclusion: Men and women with Parkinson's disease do not differ in the age of onset. Symptoms of urinary urgency and feelings of anxiety and panic are more common in women, while decreased or increased sexual desire is more common in men. Thermoregulatory dysfunction is more common in women than in men. Other nonmotor and autonomic symptoms are equally present in both genders. Keywords: